We narrowed to 7,500 results for: PAC

1. pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

   Plasmid

   #197961

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pco-vCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

   Plasmid

   #197962

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pco-nCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

   Plasmid

   #116965

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pReporter_2

   Plasmid

   #60563

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCVD438

   Plasmid

   #63759

   Purpose

   eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.

   Depositor

   James Kaper

   Article

   Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

   Insert

   eae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pERB219

   Plasmid

   #61501

   Purpose

   Const. Halotag-GFP-PACT + mCherry-DHFR

   Depositor

   Michael Lampson, David Chenoweth

   Article

   Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

   Inserts

   HaloTag-GFP-PACT
   mCherry-DHFR
   

   Use

   Tags

   HaloTag and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cas12c-sgRNA_BbsI_CAM

   Plasmid

   #183221

   Purpose

   Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. M-tdTom-NM

   Plasmid

   #48679

   Purpose

   Mammalian tdTomato activation reporter for NM with GTCCCCTCCACCCCACAGTG protospacer

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, NM-PAM, and tdTomato reporter. Compatible with N. meningitidis Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Sp6

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pFS_0143-pET30-Fusicatenibacter_saccharivorans

   Plasmid

   #116952

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

    Plasmid

    #116964

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EYFP-Sec23A

    Plasmid

    #66611

    Purpose

    YFP fusion of Sec23A

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pReporter_7

    Plasmid

    #60567

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 413-Met25 Kar2 TorsinA GFP

    Plasmid

    #32119

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

14. orthodenticle

    Plasmid

    #37285

    Depositor

    Norbert Perrimon

    Article

    Finkelstein et al Genes Dev. 1990 Sep;4(9):1516-27.

    Insert

    orthodenticle (oc Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pPP378

    Plasmid

    #158797

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-1 (D371A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pL8_3xG34-DCAF8L2_X1

    Plasmid

    #191519

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG34-DCAF8L2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pL5_3xG22-DCAF8L2_X1

    Plasmid

    #191516

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG22-DCAF8L2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJAT13araE

    Plasmid

    #18987

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    araE (araE E.coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pJAT13

    Plasmid

    #18986

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    promoter CP13
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

20. M-tdTom-ST1

    Plasmid

    #48678

    Purpose

    Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Sp6

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

    Plasmid

    #116953

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pC1300_pUB10_pcoCASphi_E9t_MCS_version2

    Plasmid

    #197950

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCI-neo-AP-4 (Myc)3-σ4

    Plasmid

    #198331

    Purpose

    Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-4 σ4
    

    Use

    Tags

    triple Myc tag

    Expression

    Mammalian

    Mutation

    silent substitution in codon 131

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST-APIC-LexAGAD

    Plasmid

    #112807

    Purpose

    gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

    Depositor

    Chun Han

    Article

    Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPP384

    Plasmid

    #158799

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-3 (D413A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pReporter_9

    Plasmid

    #60569

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHIE046 ZF43x12-C mKate2 (TUPV1)

    Plasmid

    #138726

    Purpose

    mMoClo TUPV, with ZF43x12-C promoter and mKate2 in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x12-Compact

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

    Plasmid

    #116966

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pReporter_11

    Plasmid

    #60571

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MTK2_013

    Plasmid

    #123705

    Purpose

    Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pSpacer multicistronic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pECFP-Sec23A

    Plasmid

    #66610

    Purpose

    CFP fusion of Sec23A

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pML2003

    Plasmid

    #193255

    Purpose

    Propionate sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    prpR
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Pprp and Synthetic promoter P6

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA4/TO-2xStrep-BFLF1

    Plasmid

    #162626

    Purpose

    Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pReporter_10

    Plasmid

    #60570

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG149 NeuroD 3'UTR

    Plasmid

    #12007

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N14 3'UTR (NEUROD1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA-MNase-CaURA3

    Plasmid

    #162461

    Purpose

    DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFA-MNase-CaHIS1

    Plasmid

    #162462

    Purpose

    DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pH2B-mPlum/Gag

    Plasmid

    #102255

    Purpose

    co-expression of Gag and H2B Plum in mammalian cells

    Depositor

    Suliana Manley

    Article

    Gunzenhauser et al PLoS One. 2014 Dec 10;9(12):e115095. doi

    Inserts

    Gag
    H2B Plum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. A104T DJ-1 pET15b

    Plasmid

    #60680

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    A104T DJ-1 pET15b (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A104T

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pAG186 NID1 3'UTR

    Plasmid

    #12016

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N19 3'UTR (NID1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge