8,811 results

1. mNeonGreen-2A-mTurquoise2

   Plasmid

   #98885

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mNeonGreen-2A-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLionII

   Plasmid

   #1730

   Purpose

   This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system

   Depositor

   Garry Nolan

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW-FIRE-pHLy

   Plasmid

   #170774

   Purpose

   Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   hUbC

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mScarlet-I-mTurquoise2

   Plasmid

   #98839

   Purpose

   Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mScarletI-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIN-PAmCherry-KFERQ-NE

   Plasmid

   #102365

   Purpose

   Lentiviral overexpression of PA-mCherry-KFERQ fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   KFERQ peptide
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCmini-iCAP-AAV9-X1.1

   Plasmid

   #196836

   Purpose

   non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV9-X1.1 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Tau(WT)

   Plasmid

   #187023

   Purpose

   Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

   Depositor

   George Bloom

   Article

   Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

   Insert

   MAPT (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9n(BB)-2A-GFP (PX461)

   Plasmid

   #48140

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB-cLUC

   Plasmid

   #174051

   Purpose

   Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

   Depositor

   Alberto Macho

   Article

   Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Plant

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLifeAct-mScarlet3_N1

    Plasmid

    #189767

    Purpose

    Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LifeAct-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-MET

    Plasmid

    #23889

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MET (MET Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-mRFP1

    Plasmid

    #21315

    Purpose

    MoMLV-based Retroviral vector, expresses mRFP1

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    monomeric Red Fluorescence Protein 1
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pK18msB

    Plasmid

    #177839

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

    Depositor

    Christopher Johnson

    Article

    Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2-dCas9

    Plasmid

    #112233

    Purpose

    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

    Depositor

    Thomas Gilmore

    Article

    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

    Inserts

    dCas9
    Porumycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed: Aspartic acid 10 to Glycine, Histidine 8…

    Promoter

    EFS-NS

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDisplay-BirA-ER

    Plasmid

    #20856

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2008 May . 5(5):397-9.

    Insert

    BirA-ER
    

    Expression

    Mammalian

    Mutation

    C-terminal ER targeting sequence (KDEL)

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-EGFP (BPK1098)

    Plasmid

    #133962

    Purpose

    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription

    Depositor

    Benjamin Kleinstiver

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-tTA

    Plasmid

    #104102

    Purpose

    Expresses tTA2 under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    tTA2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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