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pCBLsgRNA2

Plasmid

#199728

Purpose

Golden Gate entry vector; 2nd gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA2 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_CMV

Plasmid

#62417

Purpose

MXS_chaining vector with CMV

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJUMP42x-2A(sfGFP)

Plasmid

#126986

Purpose

Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 POLD1

Plasmid

#67128

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

POLD1 (POLD1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET15bLCFPT7

Plasmid

#53545

Purpose

Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

Depositor

Lingchong You

Article

Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

Insert

T7 RNAP

Expression

Bacterial

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B56 gamma3 shRNA 14-1

Plasmid

#10681

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma3 shRNA (PPP2R5C Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pJUMP18-merR_O

Plasmid

#127023

Purpose

Basic Part O- ORF; merR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part merR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_P2A

Plasmid

#62433

Purpose

MXS_chaining vector with P2A

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Self-cleaving peptide

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-shTRAF2#2

Plasmid

#44128

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T NHE-RF

Plasmid

#11636

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

NHE-RF (NHERF1 Human)

Tags

GST

Expression

Bacterial

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-mScarlet-TEV-Strep-His

Plasmid

#210823

Purpose

Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

mScarlet

Tags

TwinStrep, 10xHis

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFAP2B

Plasmid

#67092

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAP2B (TFAP2B Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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TOPO CFP

Plasmid

#68489

Purpose

TOPO construct expressing CFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cyan Fluorescent Protein

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10B-V5

Plasmid

#35940

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (WNT10B Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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MXS_bGpA

Plasmid

#62424

Purpose

MXS_chaining vector with bGpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

beta-Globin poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP2A13

Plasmid

#67103

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2A13 (CYP2A13 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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TL023_lenti_CMV:HaloTag-GluA1

Plasmid

#192518

Purpose

Expression of AMPA GluA1 with N terminal HaloTag fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

HaloTag-GluA1

Use

Lentiviral

Tags

HaloTag

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_hUmiRc2

Plasmid

#25747

Purpose

Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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Double UP sfGFP to mScarlet

Plasmid

#120261

Purpose

Natively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

sfGFP/mScarletP2A-mcs

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP-Hygro

Plasmid

#14686

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP and PGK-Hygro

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 RAD54B

Plasmid

#67124

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAD54B (RAD54B Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCEP4-DAT-myc

Plasmid

#107457

Purpose

Mammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Dopamine Transporter (DAT) (SLC6A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T2 (+minintr, KDR)

Plasmid

#119201

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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PP255

Plasmid

#78934

Purpose

Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJAT23

Plasmid

#204312

Purpose

For generating targeting inversions. C-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPc

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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REM2

Plasmid

#55682

Purpose

expression of mouse REM2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM2 (Rem2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pECFP-C1-rGAT

Plasmid

#70108

Purpose

mammalian expression of rat GAT with N terminal CFP fusion

Depositor

Harald Sitte

Article

Scholze et al J Biol Chem. 2002 Nov 15;277(46):43682-9

Insert

GAT (Slc6a1 Rat)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO PuroR

Plasmid

#68447

Purpose

TOPO construct expressing PuroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rab33bb in pCSDEST2

Plasmid

#140882

Purpose

Expression of zebrafish GFP-tagged Rab33bb in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab33bb

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSC130

Plasmid

#216364

Purpose

xhNup98-Nb3i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup98-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmOrange 2-Actin

Plasmid

#199703

Purpose

visualization of cleavage furrow in living mammalian mitotic cells

Depositor

Kenji Sugimoto

Article

Tanaka et al Bioimages 25: 7-19, 2017

Insert

human cytoplasmic action

Tags

mOrange 2

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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RIT1

Plasmid

#55662

Purpose

expression of mouse RIT1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RIT1 (Rit1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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RASD1

Plasmid

#55671

Purpose

expression of mouse RASD1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASD1 (Rasd1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pTW194b63

Plasmid

#136930

Purpose

Directed evolution of SpCas10

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

npuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-V5-TRAF2-puro

Plasmid

#44111

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral

Tags

V5

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pSV2-c-erbB2

Plasmid

#16250

Depositor

Mien-Chie Hung

Article

Kwong et al Mol Carcinog. 1998 Oct . 23(2):62-8.

Insert

c-erbB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO ZeoR

Plasmid

#68446

Purpose

TOPO construct expressing ZeoR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Zeocin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO HygroR

Plasmid

#68445

Purpose

TOPO construct expressing HygroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Hygromycin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1 GFP B56 alpha shRNA 6-3

Plasmid

#10680

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 alpha shRNA (PPP2R5A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
FFiBlast p5

Plasmid

#68470

Purpose

Inverted and floxed blasticidin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenLox_U6 Nlgn3

Plasmid

#59359

Purpose

Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 3 shRNA (rat) (Nlgn3 Rat)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL-neo Myr-FLAG-p110alpha

Plasmid

#10890

Depositor

William Hahn

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

p110alpha (PIK3CA Bovine)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
TOPO iRFP670

Plasmid

#68443

Purpose

TOPO construct expressing iRFP670 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

iRFP670

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Tags

FRB (T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_TagBFP

Plasmid

#62400

Purpose

MXS_chaining vector with TagBFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

TagBFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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