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We narrowed to 6,346 results for: rel

Showing: 4601 - 4650 of 6346 results

  6. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

    Plasmid
    #128698
    Purpose
    simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells
    Depositor
    Igor Weber
    Article
    Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11
    Inserts
    iqgC (full length)
    Raf1 kinase (aa 50-132)
    Use
    Dictyostelium discoideum expression vector
    Tags
    YFP and mRFP
    Expression
    Mutation
    none
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128698-plasmid-map-sequence-id-246776

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  13. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  15. pCSC-LHX8-IRES-GBX1

    Plasmid
    #182235
    Purpose
    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189
    Depositor
    Chun-Li Zhang
    Article
    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.
    Insert
    LHX8-IRES-GBX1 (LHX8, GBX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    182235-plasmid-map-sequence-id-357464

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  17. AP1muA-TurboID-polyA-G418 HR

    Plasmid
    #227734
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    Turbo-ID, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    227734-plasmid-map-sequence-id-457074

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  23. pUDP082

    Plasmid
    #103875
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103875-plasmid-map-sequence-id-199262

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  24. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  25. pLenti-Mito-Car-GECO

    Plasmid
    #100765
    Purpose
    Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Joseph Gordon
    Article
    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1
    Insert
    Lenti-Mito-Car-GECO
    Use
    Lentiviral
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100765-plasmid-map-sequence-id-193070

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  33. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

    Plasmid
    #136684
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression
    Promoter
    T7
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136684-plasmid-map-sequence-id-272124

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  36. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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  37. pUDP025

    Plasmid
    #103874
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103874-plasmid-map-sequence-id-199263

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  39. pCW57.1 KBTBD4 WT

    Plasmid
    #184624
    Purpose
    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells
    Depositor
    Vincenzo D'Angiolella
    Article
    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1
    Insert
    N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    no mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184624-plasmid-map-sequence-id-363386

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  40. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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  43. pHOT-P2Y2R

    Plasmid
    #159108
    Purpose
    entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson
    Depositor
    Fabio Mammano
    Article
    Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc
    Insert
    P2Y2R
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    UBC
    Available since
    Jan. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159108-plasmid-map-sequence-id-318664

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  46. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid
    #97044
    Purpose
    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.
    Depositor
    George Daley
    Article
    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature
    Insert
    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet on
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    97044-plasmid-map-sequence-id-192694

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Showing: 4601 - 4650 of 6346 results