We narrowed to 2,366 results for: clu

1. pCS-H2B-cerulean

   Plasmid

   #53748

   Purpose

   Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWJB9

   Plasmid

   #126650

   Purpose

   Expresses AdrA from a modified pLAFR3

   Depositor

   Ute Romling

   Article

   Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

   Insert

   adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2340_Tier3(SB)-iRFP_Puro

   Plasmid

   #169638

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-TetO-PM

   Plasmid

   #197387

   Purpose

   To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral; Teto

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS-H2B-mRFP1

   Plasmid

   #53745

   Purpose

   Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

   Plasmid

   #182344

   Purpose

   sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Spacer 4
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX-SpiCee-NES

   Plasmid

   #140901

   Purpose

   mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NES
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG5-ERRbeta2

   Plasmid

   #52186

   Purpose

   Expresses human ERRbeta2 splice variant in mammalian cells

   Depositor

   Rebecca Riggins

   Article

   Heckler et al Cell Cycle. 2014 Dec 12:0.

   Insert

   Estrogen-related receptor beta (ESRRB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type sequence; codon optimized to exclude Ec…

   Promoter

   SV40

   Available since

   April 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUASTattB-dALFANb(3mut)-mGreenLantern

   Plasmid

   #219662

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZS*11_4enh

    Plasmid

    #149423

    Purpose

    Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Inserts

    rbcS-E9 enhancer
    35S core enhancer
    Cab-1 enhancer
    AB80 enhancer
    

    Use

    Tags

    T120>A

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. cyto-YFP-FKBPx5

    Plasmid

    #103777

    Purpose

    expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-YFP-FKBPx5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCX-SpiCee-Kras

    Plasmid

    #140838

    Purpose

    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-Kras
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GG-EBNA-OSK2M2L1-PP

    Plasmid

    #102902

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OSK2M2L1-gRNAs-PGK-puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE3G-iRFP-B1-timestamp_A

    Plasmid

    #159924

    Purpose

    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB-LIC-Bse

    Plasmid

    #26108

    Purpose

    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    His6 - TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Agrp-pGL3

    Plasmid

    #17552

    Depositor

    Domenico Accili

    Article

    Kitamura et al Nat Med. 2006 May . 12(5):534-40.

    Insert

    Agrp (Agrp Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Contains ~400bp of the mouse Pomc gene promoter, …

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDM098

    Plasmid

    #216818

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold
    

    Use

    Tags

    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pdgfra-T2A-H2B-eGFP donor

    Plasmid

    #113120

    Purpose

    Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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