We narrowed to 6,267 results for: kit

1. pDGE165

   Plasmid

   #79455

   Purpose

   recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGE166

   Plasmid

   #79456

   Purpose

   recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGE169

   Plasmid

   #79459

   Purpose

   recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGE2

   Plasmid

   #79441

   Purpose

   recipient plasmid [multiplexing]; p35S:Cas9, BASTA

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2336

   Plasmid

   #67541

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBALU3

   Plasmid

   #69318

   Purpose

   Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfp

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   CFPint-FgF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBALU2

   Plasmid

   #69317

   Purpose

   Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfp

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   YFPint-FgF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pJMC-L1-Reverse-Donor-Esp3I

   Plasmid

   #197845

   Purpose

   Donor vector to restore L1 destination via Esp3I (reverse orientation)

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   None
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-C1-ADRP

   Plasmid

   #87161

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCBC-MT1T2

    Plasmid

    #50593

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MAC_C_LTK

    Plasmid

    #187762

    Purpose

    MAC-tagged gene expression of human LTK

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    LTK (LTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEFIRES-P-EGFP-ADRP

    Plasmid

    #87157

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    mCherry-R TTGGTCACCTTCAGCTTGG

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LD-B

    Plasmid

    #209861

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lyso-B

    Plasmid

    #209873

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTK3_038

    Plasmid

    #123752

    Purpose

    Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    R-FlincA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LD-GA

    Plasmid

    #209862

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cav-GA

    Plasmid

    #209880

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    CAVIN1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Red cGull

    Plasmid

    #191853

    Purpose

    Expresses biosensor for cGMP in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

    Insert

    Red cGull
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PM-B

    Plasmid

    #209876

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cyto-B

    Plasmid

    #209858

    Purpose

    Dimerization dependent fluorescent protein B fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCRISPR-PE-bacteria

    Plasmid

    #172715

    Purpose

    Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    Cas9n-linker-M-MLV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H840A of Cas9

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDestTol2pA2-U6:gRNA

    Plasmid

    #63157

    Purpose

    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBTK1009 (pSL9)

    Plasmid

    #191004

    Purpose

    Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCBC-DT3T4

    Plasmid

    #50592

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBTK1008 (pSL7)

    Plasmid

    #191003

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCBC-DT2T3

    Plasmid

    #50591

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mc2 mNeon

    Plasmid

    #206218

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTRANS_231

    Plasmid

    #91120

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Red Glifon 300

    Plasmid

    #163115

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSB700 lenti gRNA Puro

    Plasmid

    #167911

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Red Glifon 3000

    Plasmid

    #163116

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 3000
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mc2 mCherry hPGK

    Plasmid

    #206231

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Lyso-GA

    Plasmid

    #209874

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMOD_A1110

    Plasmid

    #91031

    Purpose

    Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCBC-MT3T4

    Plasmid

    #50595

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-26t plus, OsU3p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

    Plasmid

    #87693

    Purpose

    Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA-I-PhoCl-HCVp

    Plasmid

    #87697

    Purpose

    Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
    

    Use

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge