We narrowed to 13,195 results for: 109

1. pLEX307-SARS-CoV-2-3CL-C145A

   Plasmid

   #160279

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-C1 EDD

   Plasmid

   #37190

   Depositor

   Darren Saunders, Charles Watts

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD (UBR5 Human)
   

   Use

   Tags

   6x His Tag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-hLC3

   Plasmid

   #87872

   Purpose

   Express a EGFP-human MAP1-LC3 in mammalian cells

   Depositor

   Eiki Kominami, Isei Tanida

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   MAP1LC3 (MAP1LC3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CLP1

   Plasmid

   #155462

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CLP1 (CLP1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL4.23 A82V 2014 EBOV + Mucin-Like Domain

   Plasmid

   #86016

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Jeremy Luban, Pardis Sabeti

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed alanine 82 to valine

   Promoter

   CMV IE

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSG5 EDD

   Plasmid

   #37191

   Depositor

   Darren Saunders, Charles Watts

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD (UBR5 Human)
   

   Use

   Tags

   6x His Tag and FLAG

   Expression

   Mammalian

   Mutation

   K503R (please see depositor comments below)

   Promoter

   SV40

   Available since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B270 + SMARCAL1 sgSTOP

   Plasmid

   #100717

   Purpose

   B270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCerulean-PCNA-HygroR

   Plasmid

   #215064

   Purpose

   Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLEX-FHH-NRASQ61K-IRES-Puro

   Plasmid

   #120570

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hnRNPA2_LC_WT

    Plasmid

    #98657

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUb FLAG-Human IntS6L

    Plasmid

    #198409

    Purpose

    Expresses FLAG-tagged human IntS6L

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator complex subunit 6 like (INTS6L Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEX-FHH-NRAS-IRES-Puro

    Plasmid

    #120569

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. B52 + SMARCAL1 sgSTOP

    Plasmid

    #100715

    Purpose

    B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SMARCAL1 (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX307-IBV-3CL

    Plasmid

    #160288

    Purpose

    Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3 2 kb prom. and exon 1 fragment CD274

    Plasmid

    #107007

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86021

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain

    Promoter

    CMV IE

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B52 + SPRTN sgSTOP

    Plasmid

    #100709

    Purpose

    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMX-PL1-WDR5

    Plasmid

    #59970

    Purpose

    Mammalian Expression of WDR5

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Human, Synthetic)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

    Plasmid

    #132686

    Purpose

    AAV plasmid encoding mouse ACY1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Acylase I (Acy1, Acy1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-PPO-Venus

    Plasmid

    #139502

    Purpose

    Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Use

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLEX307-HCoV-NL63-3CL

    Plasmid

    #160286

    Purpose

    Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLEX-FLAG-NRASQ61K-IRES-Puro

    Plasmid

    #120575

    Purpose

    Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAPM-miR30-EIF2AK2-ts1

    Plasmid

    #174247

    Purpose

    EIF2AK2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    EIF2AK2 shRNA (EIF2AK2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLEX307-Bat-CoV-HKU9-3CL

    Plasmid

    #160284

    Purpose

    Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-NSD3-short

    Plasmid

    #72552

    Purpose

    expresses 3*FLAG tagged human NSD3-short

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HT111_Fsyn_FAS(Cre off)_QuasAr6a_Citrine

    Plasmid

    #178824

    Purpose

    Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    QuasAr6a_citrine
    

    Use

    Cre/Lox and Lentiviral

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET22b-mEB2-eGFP-6xHis

    Plasmid

    #130982

    Purpose

    Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB2-GFP-6xHis (Mapre2 Mouse)
    

    Use

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px458_2A_GFP_sgRNA_EIF2AK2

    Plasmid

    #106108

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.3−3xFLAG−AGO2(S387A)

    Plasmid

    #220462

    Purpose

    Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Human AGO2 (AGO2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Serine 387 to alanine

    Promoter

    CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX-4T1-WDR5

    Plasmid

    #59969

    Purpose

    Bacterial Expression GST-WDR5

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

32. tetO-SRF

    Plasmid

    #170704

    Purpose

    doxycycline-inducible overexpression of SRF

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLEX307-SARS-CoV-3CL

    Plasmid

    #160280

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mEGFP-N1-MST2

    Plasmid

    #166475

    Purpose

    Expresses fusion of mEGFP and MST2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, MST2 (STK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFL His-SDS22 / PP1gamma

    Plasmid

    #113511

    Purpose

    Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Inserts

    SDS22 (PPP1R7 Human)
    PP1gamma (PPP1CC Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mRuby2-Mito-7

    Plasmid

    #55905

    Purpose

    Localization: Mitochondria, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

37. CIBN-rTetR

    Plasmid

    #183919

    Purpose

    Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    CIBN-rTetR (CIB1 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mCerulean-PCNA-PuroR

    Plasmid

    #215063

    Purpose

    Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-MBP-(CBX1)3-WT

    Plasmid

    #180212

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1 x3 (CBX1 Human)
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CRAF_S259A_pcDNA3.1

    Plasmid

    #86505

    Purpose

    Expresses CRAF_S259A in mammalian cells

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S259A

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBS-f1(+)-SEC61B-mEGFP

    Plasmid

    #115910

    Purpose

    donor to insert EGFP at the SEC61B locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SEC61B-mEGFP (SEC61B Human)
    

    Use

    Donor template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFP-hGATE-16

    Plasmid

    #87870

    Purpose

    Express a EGFP-human GATE-16 in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    GATE-16 (GABARAPL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLEX-FLAG-NRAS-IRES-Puro

    Plasmid

    #120574

    Purpose

    Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. mIFP-Alpha-Actinin-19

    Plasmid

    #56210

    Purpose

    Localization: Focal Adhesions / Actin, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pHAGE2-TetO-hSOX17

    Plasmid

    #206389

    Purpose

    Expresses human SOX17 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMTS_mScarlet-H_N1

    Plasmid

    #85058

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mScarlet-H

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBItetDT240GFP

    Plasmid

    #96904

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline responsive and bidirectional

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. MBP-hnRNPA2_LC

    Plasmid

    #98661

    Purpose

    Expresses MBP-tagged hnRNPA2 AA 190-341

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. mTFP1-Farnesyl-5

    Plasmid

    #55481

    Purpose

    Localization: Plasma Membrane, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only