We narrowed to 18,760 results for: IRE;

1. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

   Plasmid

   #209084

   Purpose

   Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   NGN2 (Neurog2 Mouse)
   BirA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   hSyn

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

   Plasmid

   #157984

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-BRD9_BET-PGK-Puro-IRES-GFP

   Plasmid

   #75120

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Bromodomain replaced with the first bromodomain o…

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

4. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

   Plasmid

   #63060

   Purpose

   Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   nls-mRuby2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-C-Myc-DDK-IRES-Puro LARP7

   Plasmid

   #200725

   Purpose

   Lentiviral expression of Flag-Tagged human LARP7

   Depositor

   Claudio Alarcón

   Insert

   LARP7 (LARP7 Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

   Plasmid

   #75116

   Purpose

   Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   single amino acid mutation N216A

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

   Plasmid

   #64314

   Purpose

   chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

   Depositor

   Noriyuki Tsumaki

   Article

   Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

   Inserts

   COL11A2 promoter (COL11A2 Human)
   COL11A2 enhancer (COL11A2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and IRES-PURO

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2K-CAGGS-mouse Gfi1-IRES-GFP

   Plasmid

   #91893

   Purpose

   Mammalian expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pMXS-IRES-BLAST flag-CAD E1368A

   Plasmid

   #188141

   Purpose

   Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-BLAST flag-CAD S1396A

    Plasmid

    #188126

    Purpose

    Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB CAG Cdk6 R31C-IRES-EGFP

    Plasmid

    #153946

    Purpose

    piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    Cdk6 (Cdk6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R31C

    Promoter

    CAG

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB CAG Gfi1:FLAG-IRES-EGFP

    Plasmid

    #153945

    Purpose

    piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Chx10 (3kb proximal 5' region)-GFP-IRES-AP

    Plasmid

    #18815

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    Chx10 (3kb proximal 5' region)-GFP-IRES-AP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCSC-ASCL1-IRES-GFP-T2A-Sox11

    Plasmid

    #182233

    Purpose

    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

    Depositor

    Chun-Li Zhang

    Article

    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

    Insert

    ASCL1-IRES-GFP-T2A-Sox11
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB

    Plasmid

    #131350

    Purpose

    Expresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase B

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88-GyrB (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLK89 - Edit-directing plasmid cloning insert

    Plasmid

    #98846

    Purpose

    Contains the insert used for step 2 of edit-directed plasmid cloning

    Depositor

    Leonid Kruglyak

    Insert

    KanR
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Changed sequence to remove a MluI cut site

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-IRES-GFP-MyD88 (S34Y)-CpLxIS

    Plasmid

    #131349

    Purpose

    Expresses MyD88 S34Y with 2 copies of pLxIS motif from STING fused to its C-terminus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88 (S34Y)-CpLxIS (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    MyD88 (S34Y)

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB CAG SMARCA4 T910M-IRES-EGFP

    Plasmid

    #153949

    Purpose

    piggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T910M

    Promoter

    CAG

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid

    #135668

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgCR8
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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