165,685 results

1. pSG5-EGFP

   Plasmid

   #49246

   Purpose

   encodes enhanced green fluorescent protein (EGFP)

   Depositor

   Rebecca Riggins

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

2. HPRT

   Plasmid

   #22884

   Depositor

   James Thomson

   Article

   Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

   Insert

   hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
   

   Use

   Cre/Lox; Homologous recombination

   Mutation

   PGK, NeoR, and BGH polyA are between the two geno…

   Available Since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac HT JS-Rab10wt

   Plasmid

   #135557

   Purpose

   Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Tags

   6xHis tag, TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIBA2-Int-SpyTag

   Plasmid

   #198038

   Purpose

   Expression plasmid for producing SpyTagged stalled secretion variant of intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   lacks native signal peptide (amino acids 1-39)

   Promoter

   tet

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-7 asyn G51D

   Plasmid

   #105747

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

   Insert

   alpha-synuclein G51D (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   G51D

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SCP1-CMVenh-luc

   Plasmid

   #22216

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP1 (super core promoter 1)-CMVenh luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFD4d-U6-1:white1-U6-3:white1

   Plasmid

   #84005

   Purpose

   pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

   Depositor

   Phillip Zamore

   Article

   Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

   Inserts

   white sgRNA-1
   white sgRNA-1
   

   Use

   CRISPR

   Promoter

   U6:1, U6:3, and dU6-1; dU6-3

   Available Since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYZ183

   Plasmid

   #98425

   Purpose

   Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYZ184

   Plasmid

   #98426

   Purpose

   Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINE-3C-mCherry

    Plasmid

    #214060

    Purpose

    Expression of fluorescent fusion proteins

    Depositor

    Ray Owens

    Insert

    mCherry
    

    Tags

    Hexahistidine

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD434-SR mouse Alpha-synuclein

    Plasmid

    #89073

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pVITRO-HPV8 L1L2

    Plasmid

    #52598

    Purpose

    Expresses HPV8 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV8 L1
    HPV8 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid

    #214453

    Purpose

    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HC87 pEYFPN1 anti-Homer1 nanobody

    Plasmid

    #135223

    Purpose

    Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA-EYFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    pj23119

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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