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Plasmid#160740PurposeExpresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobodyDepositorInsertHA-nEPEA-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_1
Plasmid#103909PurposeProtein expression and purification of ZO1 PDZ1 domainDepositorInsertTJP1 (TJP1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_12xTEAD_luc2
Plasmid#173004Purposefirefly luciferase reporter vector with 12x clustered TEAD binding sitesDepositorInsert12xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK0_047
Plasmid#123977PurposeEncodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthCLYBL CAS9 Destination - HygroR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p53-(1-393)-R273H-N268D
Plasmid#35087DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-393 - R273H-N268DPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10
Plasmid#197979PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF7_P2A_Hygro_Barcode
Plasmid#120493PurposeBarcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF7 (KLF7 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-MOB1A
Plasmid#172988Purposeconstitutive expression of FLAG-tagged MOB1ADepositorInsert3xFLAG-MOB1A (MOB1A Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry
Plasmid#124493PurposeExpresses FlipYFP (TEV cleavage sequence) and T2A mCherryDepositorInsertFlipYFP(TEV cleavage seq) T2A mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK0_057
Plasmid#123950PurposeEncodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertBxBI LP on hAAVS1 (Hygro::mRuby)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal4 PGC1 alpha 1-550
Plasmid#8894DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseTagsGal4 DBDExpressionMammalianMutationamino acids 1-550 of PGC-1 alphaPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
NmeCas9_3XNLS_pMCSG7
Plasmid#120078PurposeBacterial expression of wtNmeCas9 with 3X NLSDepositorInsertNmeCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_NEUROG3_P2A_Hygro_Barcode
Plasmid#120468PurposeBarcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertNEUROG3 (NEUROG3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PL452-hGH-polyA-PGK-neoR
Plasmid#191681PurposeDonor plasmid backbone PGK-neoRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1-P2A-Hygro_Barcode
Plasmid#170221PurposeBarcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GFP
Plasmid#39290DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMV-LEU2
Plasmid#65334Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUsDepositorInsertLEU2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV E2F1 1-284
Plasmid#24216PurposeMammalian expression of human E2F1 (aa 1-284) with HA tagDepositorInsertE2F1 1-284 (E2F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
p53-(1-393)-R175H-N268D
Plasmid#35086DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-393 - R175H-N268DPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197966PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVp and pUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CCNB1-P2A-Hygro_Barcode
Plasmid#170218PurposeBarcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCCNB1 (CCNB1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK3_009
Plasmid#123724PurposeEncodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK systemDepositorInsertPHYB-VP16-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-SLC35B2-sgRNA
Plasmid#154860PurposeLentiviral expression of Cas9 and sgRNA targeting SLC35B2DepositorInsertSLC35B2 sgRNA (SLC35B2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIG-Flag-Sox9-IRES-nls-GFP
Plasmid#44281DepositorInsertFlag-Sox9 (SOX9 Chicken)
UseTagsFlagExpressionMammalianMutationPromoterCMV-IE enhancer and Chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ASCL3_P2A_Hygro_Barcode
Plasmid#120428PurposeBarcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertASCL3 (ASCL3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_015
Plasmid#123930PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - Insulator + tagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_017
Plasmid#123932PurposeEncodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertBxBI attB tagBFP destination vector
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S513A
Plasmid#186831PurposeExpresses FLAG-BMAL1-S513A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 513 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S515A
Plasmid#186832PurposeExpresses FLAG-BMAL1-S515A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 515 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S516A
Plasmid#186833PurposeExpresses FLAG-BMAL1-S516A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 516 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only