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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,039 results for: Dos;

Showing: 4851 - 4900 of 8039 results

  2. pC-BAR-YR

    Plasmid
    #61766
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    61766-plasmid-map-sequence-id-442755

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  3. pKK-CyOFP1-TEV

    Plasmid
    #105782
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    CyOFP1-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105782-plasmid-map-sequence-id-203371

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  5. pKK-mClover3-TEV

    Plasmid
    #105778
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mClover3-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105778-plasmid-map-sequence-id-203367

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  8. pHR_PGK_antiCD19_eZ3_Gal4VP64

    Plasmid
    #169917
    Purpose
    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.
    Depositor
    Zhili Rong
    Article
    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do
    Insert
    antiCD19-Notch3-GL4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Additional EGF repeat between the extracellular d…
    Promoter
    PGK
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169917-plasmid-map-sequence-id-336047

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  11. pcDNA3-Flag-Tet2 CD Mut

    Plasmid
    #72220
    Purpose
    Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 2 (Tet2 Mouse)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET2 catalytic domain (cata…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pKK-TEV-mVenus

    Plasmid
    #105791
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105791-plasmid-map-sequence-id-203382

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  19. pBT3000

    Plasmid
    #122536
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::tram-1 (tram-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122536-plasmid-map-sequence-id-238187

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  23. pKK-TEV-ProteinA

    Plasmid
    #105788
    Purpose
    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-ProteinA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105788-plasmid-map-sequence-id-203379

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  27. pBT3011

    Plasmid
    #122539
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::cup-5 (cup-5 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122539-plasmid-map-sequence-id-238204

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  29. pBT3003

    Plasmid
    #122537
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::pisy-1 (pisy-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122537-plasmid-map-sequence-id-238215

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  32. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  35. pBT3014

    Plasmid
    #122540
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::rab-7 (rab-7 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122540-plasmid-map-sequence-id-238121

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  36. pPBC-LG3-tdT

    Plasmid
    #62810
    Purpose
    pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62810-plasmid-map-sequence-id-123118

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  37. Human a3 nicotinic receptor subunit EM construct

    Plasmid
    #163960
    Purpose
    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Asn348-Ser402 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163960-plasmid-map-sequence-id-323882

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  41. pLS2965

    Plasmid
    #122534
    Purpose
    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_aman-2::CemOrange2 (aman-2 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122534-plasmid-map-sequence-id-238120

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  44. pBT3024

    Plasmid
    #122542
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_mt::CemOrange2
    Use
    Tags
    MLSLRQSIRFFKPATRTLCSSRTLL
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122542-plasmid-map-sequence-id-238125

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  45. pBT3007

    Plasmid
    #122538
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::rab-5 (rab-5 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122538-plasmid-map-sequence-id-238129

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  46. pBT3037

    Plasmid
    #122544
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122544-plasmid-map-sequence-id-238132

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  47. pKK-MBP-TEV

    Plasmid
    #105771
    Purpose
    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    MBP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105771-plasmid-map-sequence-id-203360

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Showing: 4851 - 4900 of 8039 results