We narrowed to 4,989 results for: puc
-
Plasmid#176258PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationH840A and D10A mutations on spCas9 to inactivate …PromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a_crRNA Td2
Plasmid#176255PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationD917A and E1006A mutations to inactivate the endo…PromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU002
Plasmid#174299PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHA and cMycExpressionYeastMutationN343Q, N501YPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU001
Plasmid#174298PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHA and cMycExpressionYeastMutationN343Q, E484KPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ir7f-T2A-QF2 HDR plasmid
Plasmid#140942PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f geneDepositorInsertIr7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
UseTagsExpressionMutation4 point mutations (annotated on plasmid map) were…PromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)5
Plasmid#127523PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)7
Plasmid#127524PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)9
Plasmid#127525PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)2
Plasmid#127521PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Empty Bridge Control Zfp462-Klf4SE
Plasmid#127666PurposeEncodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.DepositorInsertgRNAs 129, 135, 115, 117
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAP1893-1
Plasmid#105248Purposehuman GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)DepositorInsertInsertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV2A
Plasmid#224440PurposeRep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDQTTL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-23:TJP1-mEGFP
Plasmid#87429PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1DepositorInsertTJP1 Homology Arms with mEGFP-linker (TJP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
kiCAP-AAV-MyoAAV4A
Plasmid#224448PurposeRep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDYNSL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV1A
Plasmid#224437PurposeRep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDLTTP insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV3A
Plasmid#224442PurposeRep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDYVGL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP
Plasmid#207862PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4E
Plasmid#224452PurposeRep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDFNNT insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV1E
Plasmid#224439PurposeRep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDTMSK insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-24:MYH10-mEGFP
Plasmid#87428PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10DepositorInsertMYH10 Homology Arms with mEGFP-linker (MYH10 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
kiCAP-AAV-MyoAAV3F
Plasmid#224447PurposeRep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDHASW insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-1:PXN-EGFP
Plasmid#87420PurposeHomology arms and linker-EGFP sequence for C-terminus tagging of human PXNDepositorInsertPXN Homology Arms with linker-EGFP (PXN Human)
UseCRISPR; Donor templateTagslinker-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
kiCAP-AAV-MyoAAV1C
Plasmid#224438PurposeRep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDLSTP insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB
Plasmid#207859PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a-KRAB_crRNA NC
Plasmid#176262PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleasDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsKruppel associated box domain and luciferaseExpressionMutationD917A and E1006A mutations to inactivate the endo…PromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9-KRAB_sgRNA Td2
Plasmid#176264PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsKruppel associated box domain and luciferaseExpressionMutationH840A and D10A mutations on spCas9 to inactivate …PromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
CYLBL_Neo_CAGdCas9-KRAB
Plasmid#220439PurposeCAG-dCas9-KRAB knock-in using TALEN at CLYBL locusDepositorInsertdCas9
UseCRISPR and TALENTagsKRABExpressionMammalianMutationD10A and H840APromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV2E
Plasmid#224441PurposeRep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDQGYQ insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV3D
Plasmid#224445PurposeRep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDYREL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV3E
Plasmid#224446PurposeRep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDHGVL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4B
Plasmid#224449PurposeRep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDYTSV insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4D
Plasmid#224451PurposeRep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDHGVL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4C
Plasmid#224450PurposeRep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDYTSM insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV3C
Plasmid#224444PurposeRep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDYSSV insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV3B
Plasmid#224443PurposeRep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDYSGL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX-N-CTCF-mAID-Halo-donor
Plasmid#139747PurposeMouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTagDepositorInsertCTCF CRISPR knock-in (Ctcf Mouse)
UseCRISPRTagsmAID-HaloTagExpressionMutationPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTR-469 JUNB-tNGFR
Plasmid#186060PurposeKnockin of truncated NGFR to target geneDepositorInsertJUNB (JUNB Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-472 FOXO1-tNGFR
Plasmid#186062PurposeKnockin of truncated NGFR to target geneDepositorInsertFOXO1 (FOXO1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pvalb-2A-Flpo Flp-in replacement vector
Plasmid#61572PurposeRecombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codonDepositorInsertPvalb-2A-Flpo
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1595
Plasmid#121062PurposemKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmKate2^SEC^BioTag::AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…ExpressionWormMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTR-374 tNGFR-P2A-B2M
Plasmid#186068PurposeKnockin of truncated NGFR to target geneDepositorInsertB2M (B2M Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tre-Tight ifgMosaic Donor vector (LH121)
Plasmid#99630PurposeTo clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/LoxTagsExpressionMutation(Please see depositor comments below)PromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Target Zfp462-Klf4SE
Plasmid#127668PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1592
Plasmid#121059PurposeGFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertGFP^SEC^BioTag::AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…ExpressionWormMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTR-471 IRF4-tNGFR
Plasmid#186061PurposeKnockin of truncated NGFR to target geneDepositorInsertIRF4 (IRF4 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bxb1Donor_G102H_ADRB2_LucReporter
Plasmid#129794Purposeencodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter geneDepositorInsertADRB2 (ADRB2 Human)
UseSynthetic BiologyTags3x FLAGExpressionMammalianMutationG102HPromoterN/AAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S(rc)2_4_5-GFP
Plasmid#127520PurposePlasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.DepositorInsertCaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only