8,811 results

1. pET-BiFC

   Plasmid

   #87856

   Purpose

   pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   Encodes for MCS & C-term half of mVenus
   Encodes for MCS & N-term half of mVenus
   

   Tags

   BiFC C-terminal fragment

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-puro/Flag PD-L1 WT

   Plasmid

   #121446

   Purpose

   expression of huamn PD-L1 wildtype in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFlagCMV2-EFP

   Plasmid

   #12449

   Depositor

   Dong-Er Zhang

   Article

   Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

   Insert

   EFP (TRIM25 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-BirA*-dCas9-eGFP

   Plasmid

   #84779

   Purpose

   Expresses BirA*-dCas9-eGFP in mammalian cells.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   BirA*-dCas9-eGFP
   

   Use

   CRISPR

   Tags

   cMyc

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   July 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-C3-Sec5

   Plasmid

   #53756

   Purpose

   pEGFP-C3 plasmid that expresses Sec5

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Sec5 (EXOC2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. scFLARE2

   Plasmid

   #158700

   Purpose

   Neuronal expression of scFLARE2 component

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   scFLARE2
   

   Use

   AAV

   Tags

   HA, Flag, VP16

   Expression

   Mammalian

   Mutation

   Truncated form of TEV protease (220-242 amino aci…

   Promoter

   Syn

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAVS1-PDi-CRISPRn

   Plasmid

   #73500

   Purpose

   Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   Cas9
   rtTA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CAG and TRE

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2+mNeonGreen-C Cloning Vector

   Plasmid

   #128144

   Purpose

   To express a protein of interest fused to the C-terminus of mNeonGreen

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   mNeonGreen fusion

   Expression

   Mammalian

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA GSK3 beta S9A pcDNA3

   Plasmid

   #14754

   Purpose

   Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

   Depositor

   Jim Woodgett

   Article

   Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

   Insert

   GSK3 beta S9A (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S9A. Constitutively active mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Human MAC-1 alpha

    Plasmid

    #8631

    Depositor

    Timothy Springer

    Article

    Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

    Insert

    MAC-1 alpha (ITGAM Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pAAV.CAG.GCaMP6f.WPRE.SV40

    Plasmid

    #100836

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE2 Lnp-mCherry

    Plasmid

    #86687

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    LNPK (LNPK Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ubiquitin WT

    Plasmid

    #12647

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubiquitin - wild type (UBC Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC18T-mini-Tn7T-Gm-ecfp

    Plasmid

    #65030

    Purpose

    GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDx_mScarlet-I3

    Plasmid

    #189757

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5 beta4

    Plasmid

    #16037

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMK287 (mAID-Hygro)

    Plasmid

    #72825

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Hygro
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAW12.lentiguide.GFP

    Plasmid

    #104374

    Purpose

    GFP vector for cloning in guides

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge