19,185 results

1. HBV 1.3-mer X-null replicon

   Plasmid

   #65461

   Purpose

   containing 1.3 units of the X-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

   Insert

   1.3 units of the X-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

   Plasmid

   #85225

   Purpose

   Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

   Depositor

   John Naughton

   Inserts

   Optimized G Protein
   Enhanced GFP
   TVA (CD320 Chicken)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-ZEAXipi

   Plasmid

   #53287

   Purpose

   Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   crtE, idi, crtY, crtI, crtB, crtZ
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro IRES-EGFP

   Plasmid

   #14430

   Depositor

   L. Miguel Martins

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-CRY2-5ptaseOCRL

   Plasmid

   #66836

   Purpose

   Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   5ptaseOCRL (OCRL Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAKgfplux1

   Plasmid

   #14083

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CMV-CFP;UBC-Cre25nt

   Plasmid

   #65727

   Purpose

   Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

   Depositor

   Jacco van Rheenen

   Article

   Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

   Inserts

   CMV promoter driving CFP expression
   UBC promoter driving Cre-25nt expression
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and UBC

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAGGS-EGFP

   Plasmid

   #22212

   Purpose

   AAV zinc finger donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   CAGGS-eGFP
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-N-Myc_C223S USP7

   Plasmid

   #131243

   Purpose

   Mammalian expression of catalytically inactive Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 cyst…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSB-HYG

    Plasmid

    #166699

    Purpose

    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    adh15 promoter and tRNA promoter

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

    Plasmid

    #164458

    Purpose

    AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPR-EGFP

    Plasmid

    #75159

    Purpose

    Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL2-NOS2Promoter-Luciferase

    Plasmid

    #19296

    Depositor

    Charles Lowenstein

    Article

    Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

    Insert

    NOS2 Promoter (Nos2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM1

    Plasmid

    #51401

    Purpose

    Expresses truncated version of NLL-MetRS from endogenous MetG promoter

    Depositor

    David Tirrell

    Article

    Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

    Insert

    MetRS (metG E coli)
    

    Use

    Tags

    Expression

    Mutation

    L13N, Y260L, H301L; Please note that a truncated …

    Promoter

    MetG

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn1-FLEX-CybSEP2-WPRE

    Plasmid

    #207661

    Purpose

    Monitoring neuropeptide release

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Cytochrome b-561 (Cyb561 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Histidine 86 and 159 to alanines, inserted 2 X SE…

    Promoter

    hSynapsin

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLifeAct-mScarlet3_N1

    Plasmid

    #189767

    Purpose

    Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LifeAct-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-mCherry-flex-dtA

    Plasmid

    #58536

    Purpose

    Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

    Depositor

    Naoshige Uchida

    Article

    Wu et al Nature. 2014 May 15;509(7500):325-30. do

    Insert

    dtA (DIP_RS18250 )
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. Mucolipin1-pEGFP C3

    Plasmid

    #62960

    Purpose

    full length human Mucolipin-1 cloned into pEGFP C3

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

22. MSCV-AML1/ETO-IRES-GFP

    Plasmid

    #60832

    Purpose

    AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    AML1/ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSBbi-pur H-2Kb

    Plasmid

    #111623

    Purpose

    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

    Depositor

    Jon Yewdell

    Insert

    H2Kb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. phNFL

    Plasmid

    #132589

    Purpose

    Expresses human NFL

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3.1-NEFH

    Plasmid

    #26978

    Depositor

    David Sidransky

    Article

    Kim et al PLoS One. 2010 . 5(2):e9003.

    Insert

    NEFH (NEFH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3 flag p53

    Plasmid

    #10838

    Depositor

    Thomas Roberts

    Article

    Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

    Plasmid

    #89608

    Purpose

    Expression of Luciferase in human cells. 3rd generation lentiviral vector

    Depositor

    Fernando Fierro

    Article

    Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MNDU3

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

    Plasmid

    #159128

    Purpose

    expresses KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-FlagMLL-pl-ENL(5613)

    Plasmid

    #20873

    Depositor

    Robert Slany

    Article

    Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

    Insert

    MLL (KMT2A, MLLT1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge