We narrowed to 51,027 results for: des.1

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lentiCRISPR v2-sgBAX-1

Plasmid

#210013

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Flag-LplA

Plasmid

#232118

Purpose

For bacterial expression of Flag-tagged E. coli LplA.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

lplA (lplA Escherichia coli (strain K12))

Tags

Flag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -1

Plasmid

#198419

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgSMARCA4 guide 1

Plasmid

#193604

Purpose

SMARCA4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSMARCA4 guide 1 (SMARCA4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Met1 diUb (1-152)

Plasmid

#110766

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKIV(1-313)

Plasmid

#45063

Purpose

Expression of constit active CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV(1-313) (Camk4 Mouse)

Expression

Mammalian

Mutation

truncated at amino acid 313*

Promoter

RSV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-Nkx2-1*/Neo

Plasmid

#31272

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

Nkx2-1* (Nkx2-1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-1

Plasmid

#133353

Purpose

human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRK-ATF4 1-275

Plasmid

#26118

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF4 (ATF4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

aa's 276-351 have been deleted

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only
gRNA-hIRF-1 #12

Plasmid

#61079

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

Insert

gRNA_hIRF1 promoter #12 (IRF1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Paragranulin+linker 1

Plasmid

#176915

Purpose

express Paragranulin with linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin + linker 1 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.1.mKO2

Plasmid

#85209

Purpose

TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTsc1#1/Cre

Plasmid

#173657

Purpose

Expresses a Tsc1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tsc1 (Tsc1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#1/Cre

Plasmid

#173647

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 1+linker2

Plasmid

#176918

Purpose

express Granulin 1 with linker 2 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 1+linker2 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

Plasmid

#107729

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.1_puro_shJUND #1

Plasmid

#136581

Purpose

Expresses an inducible short hairpin targeting human JUND sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shJUNDv1 (JUND Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCGT-HCF-1-C600

Plasmid

#99364

Purpose

C-term of HCFC1 protein

Depositor

Winship Herr

Article

Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

Insert

HCF-1 C-term 1436-2035 (HCFC1 Human)

Tags

2x tandem T7 tag and C-myc

Expression

Mammalian

Mutation

Deletion of HCFC1 cDNA

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNf2#1/Cre

Plasmid

#173643

Purpose

Expresses a Nf2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf2 (Nf2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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