Showing: 81 - 100 of 142695 results
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Plasmid#59702PurposeThis lentiviral vector can be used to assay Cas9 activity.DepositorInsertEGFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-EcoBam
Plasmid#59705PurposeFolding reporter with ampicillin resistance as a readoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSALECT-Ham1
Plasmid#59706PurposepSALECT with Ham1 insertDepositorInsertHam1
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSALECT-Efnb2
Plasmid#59707PurposepSALECT with Efnb2 insertDepositorInsertEfnb2
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSALECT-AhyI
Plasmid#59708PurposepSALECT with AhyI insertDepositorInsertAhyI
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSALECT-PRAI
Plasmid#59709PurposepSALECT with PRAI insertDepositorInsertPRAI
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSALECT-SupH
Plasmid#59710PurposepSALECT with SupH insertDepositorInsertSupH
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingTagsExpressionMutationPromotermNanogAvailabilityAcademic Institutions and Nonprofits only -
Human NANOS3-P2A-mCherry knock-in targeting construct
Plasmid#59721PurposeGene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.DepositorInsertP2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FOR human NANOS3 TALEN
Plasmid#59722PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertHD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
REV human NANOS3 TALEN
Plasmid#59723PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertNG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
Human BLIMP1 sgRNA
Plasmid#59724PurposePlasmid encoding sgRNA to generate BLIMP1 knockout mutant human cellsDepositorInserthBLIMP1 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Human SOX17 sgRNA
Plasmid#59725PurposePlasmid encoding sgRNA to generate SOX17 knock-out mutant human cellsDepositorInserthSox17 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Human T (BRACHYURY) sgRNA
Plasmid#59726PurposePlasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cellsDepositorInserthT sgRNA (TBXT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiTagsExpressionMutationPromoteropposing T7 promotersAvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PSG
Plasmid#59731PurposeExpresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteriaDepositorInsertTJP1 (TJP1 Human)
UseTagsHIS and TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p221- purVSG117UTR
Plasmid#59732PurposeInserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.DepositorInsertVSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
UseFor homologous recombination into vsg221 expressi…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBSVSG221KOblast
Plasmid#59733PurposeFor replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.DepositorInsertVSG221 knockout construct
UseFor homologous recombination into vsg221 expressi…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ2
Plasmid#59734Purposeexpresses PDZ2 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 PDZ2 (TJP1 Human)
UseTagsHIS and TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_PDZ2
Plasmid#59735Purposeexpresses PDZ2 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsGST and thrombin clvgExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 142695 results