Showing: 21 - 40 of 1613 results
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Plasmid#48962Purposeto drive the sgRNA expression under a U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only
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pDD162 (Peft-3::Cas9 + Empty sgRNA)
Plasmid#47549PurposeCas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.DepositorInsertsCas9
Empty sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
Plasmid#47550PurposeCas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion alleleDepositorInsertsCas9
ttTi5605 sgRNA
UseCRISPRTagsHA and NLSExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterU6 and eef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pDD104 (Peft-3::Cre)
Plasmid#47551PurposeCre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germlineDepositorInsertCre Recombinase
UseCre/LoxTagsExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
SP6-sgRNA-scaffold
Plasmid#47912PurposeScaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injectionDepositorTypeEmpty backboneUseCRISPRTagssgRNA 3' endExpressionWormMutationPromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
pMB60
Plasmid#47941PurposeCan be used to generate synthetic guide in vitro from T7 promotorDepositorInsertsynthetic guide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMB70
Plasmid#47943PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequencesDepositorInsertguide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMB62
Plasmid#47944PurposeExpresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pMB63
Plasmid#47945PurposeExpresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pMB66
Plasmid#47946PurposeExpresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormMutationPromoterhsp-16.48AvailabilityAcademic Institutions and Nonprofits only -
pMB67
Plasmid#47947PurposeExpresses C. elegans optimized Cas9 from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromoterhsp-16.48AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1655
Plasmid#51481PurposeminiMos transposable element with HygroR, R4-R3 Gateway cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1662
Plasmid#51482PurposeminiMos transposable element with HygroR, MCS cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1656
Plasmid#51483PurposeminiMos transposable element with HygroR and peel-1, R4-R3 Gateway cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1663
Plasmid#51484PurposeminiMos transposable element with HygroR and peel-1, MCS cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1666
Plasmid#51485PurposeminiMos transposable element with PuroR, MCS cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOG2002
Plasmid#49514PurposeTALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegansDepositorTypeEmpty backboneUseTags3xFLAG, FokI-AS, and SV40 NLSExpressionWormMutationPromoterhsp-16.2AvailabilityAcademic Institutions and Nonprofits only -
pOG2003
Plasmid#49516PurposeTALEN backbone for expression of TALE(deltaRDV)-FokI RR in C. elegansDepositorTypeEmpty backboneUseTagsFokI-RR, HA, and SV40NLSExpressionWormMutationPromoterhsp-16.2AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
UseTagsGFP (S65C)ExpressionWormMutationPromotersur-5AvailabilityAcademic Institutions and Nonprofits only -
Showing: 21 - 40 of 1613 results