1,737 results

1. PGK-EGFP-FKBPF36V

   Plasmid

   #199765

   Purpose

   plasmid for targeting dTAG into the AAVS1 locus

   Depositor

   Hannes Lans

   Article

   van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

   Insert

   GFP-dTAG
   

   Use

   Tags

   GFP and dTAG

   Expression

   Mammalian and Worm

   Mutation

   Promoter

   hPGK

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmec-4::Chrimson::SL2::mCherry::unc-54

   Plasmid

   #107745

   Purpose

   Express Chrimson and mCherry in mechanosensory neurons.

   Depositor

   Andrew Leifer

   Article

   Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

   Inserts

   Chrimson
   mCherry
   

   Use

   Tags

   Expression

   Insect and Worm

   Mutation

   Promoter

   mec-4

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

   Plasmid

   #159816

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCFJ782 - HygroR

   Plasmid

   #190933

   Purpose

   Hygromycin resistance co-injection marker

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Hygromycin resistance gene
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-0

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

   Plasmid

   #159807

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDD162 (Peft-3::Cas9 + Empty sgRNA)

   Plasmid

   #47549

   Purpose

   Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   Empty sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSEM318 - sgRNA mosTI unc-119 single-copy

   Plasmid

   #159822

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 single-copy
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSF11-wmTagBFP2

   Plasmid

   #184934

   Purpose

   Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   tag-168

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAS1-Prig-3::tagBFP

   Plasmid

   #165598

   Purpose

   rig-3 promoter derive BFP expression in AVA head neurons.

   Depositor

   Andrew Leifer

   Article

   Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

   Insert

   Prig-3, tagBFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFPmAID

    Plasmid

    #174213

    Purpose

    GFP::mAID donor backbone

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFJ90 - Pmyo-2::mCherry::unc-54utr

    Plasmid

    #19327

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-2::mCherry
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

    Plasmid

    #159899

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

    Plasmid

    #159796

    Purpose

    Histamine based negative selection marker to paralyze animals with arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

    Insert

    pEXP[Prpl-3 | histamine | rpl-3 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHW394 (15xUAS::GFP::let-858 3'UTR)

    Plasmid

    #85584

    Purpose

    cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    15xUAS-delta pes-10-GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    GFP(S65C) with three introns for expression in C.…

    Promoter

    15xUAS-delta pes-10-GFP

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

    Plasmid

    #182357

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

    Plasmid

    #182353

    Purpose

    GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMDJ231 - Phsp Cas9

    Plasmid

    #191382

    Purpose

    Heat shock inducible Cas9 expression in C. elegans.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Phsp-16.41 Cas9 gpd-2 tagRFP-t
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Phsp-16.41

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSM-GFPnovo2

    Plasmid

    #116943

    Purpose

    GFPnovo2_unc-54 utr vector for expression in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Hendi et al MicroPubl Biol. 2018 Sep 6;2018:10.17912

    Insert

    C. elegans codon optimized GFPnovo2
    

    Use

    Tags

    GFPnovo2

    Expression

    Worm

    Mutation

    Y145F, V163A, S202T, L221V

    Promoter

    none

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge