Addgene

Showing: 21 - 40 of 1527 results

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pMyo-2::oxCerulean-oxVenus::Lgg-1

Plasmid

#68043

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the pharynx, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-2::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyo-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68044

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-3::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pRab-3::Cerulean-Venus::Lgg-1

Plasmid

#68045

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pRab-3::Cerulean-Venus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Gatewayp221oxCerulean-oxVenus

Plasmid

#68047

Purpose

oxCerulean-oxVenus in Gateway [1-2] plasmid p221

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

p221oxCerulean-oxVenus

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEft-3::Cerulean-Venus

Plasmid

#68048

Purpose

Ubiquitious expression of oxCerulean-oxVenus (dFP)

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Availability

Academic Institutions and Nonprofits only
Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Availability

Academic Institutions and Nonprofits only

Enlarge
pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Use

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AP56-5

Plasmid

#65591

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
AP56-6

Plasmid

#65593

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
AP56-7

Plasmid

#65594

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
AP56-8

Plasmid

#65596

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa13

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
AP54-3

Plasmid

#65597

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
AP54-5

Plasmid

#65599

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
pIK198

Plasmid

#65629

Purpose

"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pIK208

Plasmid

#65630

Purpose

sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pIK127

Plasmid

#65631

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pIK137

Plasmid

#65632

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Punc-4::NGL-1::spGFP1-10

Plasmid

#65827

Purpose

For trans-synaptic labeling

Depositor

Kang Shen, Cori Bargmann

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Use

Tags

spGFP1-10

Expression

Worm

Mutation

Promoter

Punc-4

Availability

Academic Institutions and Nonprofits only

Enlarge

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