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Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
UseTagsparS segmentsExpressionYeastMutationPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCM184-ParB2::eGFP
Plasmid#87252PurposeExpresses ParB2-EGFP in yeast. Also known as pOR2-GFPDepositorInsertParB2
UseTagsEGFPExpressionYeastMutationPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
UseTagsExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT0127
Plasmid#8728DepositorInsertyEGFP
UseTagsCodon optimized linkerExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM82
Plasmid#133648PurposeIntegration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorInsertp1k-NAB2-mCherry (NAB2 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM119
Plasmid#133661PurposeIntegration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorInsertp1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8644
Plasmid#126919PurposePlasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYBDepositorInsertXdCrtI and HMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG2J
Plasmid#102882PurposeExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertsOsTIR1
mGFP-AID
UseTagsAID(71-114)ExpressionYeastMutationPromoterADH1 and TEF1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB8787
Plasmid#126921PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1DepositorInsertHMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8748
Plasmid#126918PurposePlasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtEDepositorInsertXdCrtE
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG
Plasmid#176395PurposeExpresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.DepositorInsertGAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a PhoCl-TsCC(B)::KANMX
Plasmid#185906PurposePlasmid for cloning PhoCl fused to TsCC(B) into yeast.DepositorInsertPhoCl-TsCC(B)
UseTagsPhoCl fluorophore with C-terminal TsCC(B)ExpressionYeastMutationPromoterSP6Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2
Plasmid#41843DepositorInsertCD20
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-Kan
Plasmid#44900PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only