Addgene

Showing: 61 - 80 of 2203 results

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pIS1 PELO

Plasmid

#60804

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains PELO 3' UTR and wild-type miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

PELO 3'UTR and wild-type miR-155 binding site (PELO Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

thymidine kinase (HSV-TK promoter)

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS1 mPELO

Plasmid

#60805

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains PELO 3' UTR and mutated miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

PELO 3'UTR and mutated miR-155 binding site (PELO Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutated miR-155 binding site

Promoter

thymidine kinase (HSV-TK promoter)

Availability

Academic Institutions and Nonprofits only

Enlarge
CIP2Aprom1802bp-pGL4.10Luc

Plasmid

#60868

Purpose

Luc reporter driven by 1802 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

1802 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
CIP2Aprom865bp-pGL4.10Luc

Plasmid

#60869

Purpose

Luc reporter driven by 865 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

865 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CIP2Aprom417bp-pGL4.10Luc

Plasmid

#60870

Purpose

Luc reporter driven by 417 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

417 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CIP2Aprom392bp-pGL4.10Luc

Plasmid

#60871

Purpose

Luc reporter driven by 392 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

392 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CIP2Aprom335bp-pGL4.10Luc

Plasmid

#60872

Purpose

Luc reporter driven by 335 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

335 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CIP2Aprom285bp-pGL4.10Luc

Plasmid

#60873

Purpose

Luc reporter driven by 285 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

285 bp promoter fragment of Cancerous Inhibitor of PP2A2 (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CIP2Aprom204bp-pGL4.10Luc

Plasmid

#60874

Purpose

Luc reporter driven by 204 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

204 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CIP2Aprom108bp-pGL4.10Luc

Plasmid

#60875

Purpose

Luc reporter driven by 108 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

NM_020890108 bp promoter fragment of Cancerous Inhibitor of PP2A.2 (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CIP2Aprom27bp-pGL4.10Luc

Plasmid

#60876

Purpose

Luc reporter driven by 27 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

27 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
ErbB-3-pGL3

Plasmid

#60899

Purpose

human ErbB-3 promoter reporter construct

Depositor

Frederick Domann

Article

Zhu et al Breast Cancer Res Treat. 2002 Jan;71(1):

Insert

ErbB-3 promoter (ERBB3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

Plasmid

#62576

Purpose

Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Expression

Mutation

miR-206 binding site mutated (ACATTCCA --> AAC…

Promoter

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

Plasmid

#62577

Purpose

Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

Plasmid

#62579

Purpose

Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

Plasmid

#62580

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Tags

Expression

Mutation

miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

Promoter

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

Plasmid

#62581

Purpose

Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Tags

Expression

Mutation

miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

Promoter

Availability

Academic Institutions and Nonprofits only
CMV-LUC2CP/ARE

Plasmid

#62857

Purpose

Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

Depositor

Gideon Dreyfuss

Article

Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

destabilizing sequences (DS) added to the C termi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-LUC2CP/intron/ARE

Plasmid

#62858

Purpose

splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

Depositor

Gideon Dreyfuss

Article

Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

insertion of chimeric intron at nueotide poclsiti…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge

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