Showing: 41 - 60 of 385 results
-
Plasmid#24578DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationLysine-Aspartate-Aspartate 359-361 changed to Phe…PromoterAvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-3XKD/Y1699C
Plasmid#25367DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pLHCX-gpASNase1
Plasmid#121526PurposeExpresses gpASNase1 in mammalian cells.DepositorInsertgpASNase1
UseRetroviralTagsFLAGExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148D/F149D)
Plasmid#71388PurposeExpression of hUCH37 (M148D/F149D) in E.coliDepositorInserthUCH37 (ISF1) M148D/F149D (UCHL5 Human)
UseTagsHIS-TEV sequenceExpressionBacterialMutationchanged methionine 148 to aspartate, phenylalanin…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc rat S-SCAM alpha
Plasmid#40213DepositorInsertS-SCAM (Magi2 Rat)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKeima-LiveDrop
Plasmid#199695Purposelipophagy reporter system based on the Keima-fluorophoreDepositorInsertGPAT4 (GPAT4 Human)
UseTagsKeimaExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
PMXS-SLC1A3
Plasmid#72873PurposeThe retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson AssemblyDepositorInsertSLC1A3 (SLC1A3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
YFP-synDAT-S/D
Plasmid#48793PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygroDepositorInsertYFP-synhDAT-S/D (SLC6A3 Human)
UseTagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-DCX(S306D)-2A-mCherry
Plasmid#118292PurposeExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acidDepositorInsertDoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationSerine 306 changed to Aspartic AcidPromoterCMV with beta global intronAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-V5H6-mPCSK3 D153A
Plasmid#122681PurposeExpresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)DepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
UseTagsExpressionMammalianMutationchanged Aspartic Acid 153 to AlaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-GFP-Numb-S276D
Plasmid#133752PurposeNumb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLDDepositorInsertNUMB endocytic adaptor protein (Numb Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationserine 276 converted to aspartic acidPromoterEF1-alphaAvailabilityAcademic Institutions and Nonprofits only -
pTwist-SFFV-NFE2L18ND-Puro
Plasmid#181918PurposeLentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutation8 putative N-glycoyslation sites converted from a…PromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-3XKD
Plasmid#25049DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorUseTagsExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DED/AAA USP7
Plasmid#131253PurposeMammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationContains point mutations that convert USP7 aspart…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBK086
Plasmid#183145PurposepET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expressionDepositorInsertMapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBK092
Plasmid#183146PurposepET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expressionDepositorInsertCrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
PMXS-GOT1
Plasmid#72872PurposeThe retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.DepositorInsertGOT1 (GOT1 Human)
UseRetroviralTagsExpressionMammalianMutationsgRNA resistant human GOT1, 7 silent mutations c1…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-3XKD-R1441C
Plasmid#25050DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-3XKD-Y1699C
Plasmid#25051DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 385 results