We narrowed to 1,042 results for: URE

... Depositor Collections MDH CUREs Community Malate Dehydrogenase CUREs Community...Research Experiences (CUREs) (Link opens in a new window) The Malate Dehydrogenase CUREs Community (MCC) (...hub of educators teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional hub...begin a sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and ...Resources MDH CUREs Community (Link opens in a new window) — Learn how to get started with a CURE, explore ...experience (CURE) projects. Depositor...Course-based Undergraduate Research Experiences (CUREs) centered on a single enzyme, malate dehydrogenase...

...downstream targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8)...

...addition of a structured motif to enhance pegRNA stability PEmax — optimized PE architecture; increased ...synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries...highest levels of single-gene activation (Figure 9D) Figure 9: Overview of CRISPRi/a. In bacteria, activating...Berkeley. Plan Your CRISPR Experiment Resources Figures All figures were created using BioRender.com (Link opens..., N., Ishitani, R., Zhang, F., & Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA...vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. PMID: 25830891 Rauch...changing the target sequence present in the gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR...

...isolated and placed in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is...Antibodies can also be produced in cell cultures using hybridomas, cell cultures created by fusing B cells with...form a “Y” shape, as shown in Figure 1. The two arms of the Y structure are responsible for binding the...Monoclonal antibodies are generated from cultured B cells, where each culture should produce the same antibody...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared... a signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary ...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...

...Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements of membrane...highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide...Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-gTEMP Intracellular Heat...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays....enable investigation of a signaling pathway or measurement of a biomolecule while largely preserving spatial...fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300. Douglas Kim , GENIE...

...DNA and cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II ...vivo genome editing using Staphylococcus aureus Cas9. Nature . 520(7546):186-91. PMID: 25830891 Savic ... phages but also to destroy the phages upon re-exposure. More specifically, specialized Cas proteins snip...are grouped into two large classes based on the structure of the effector complex that cleaves the genomic... immune system seems like a winning strategy to ensure prokaryotic survival, but it is not invincible.... CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more than 6,300... loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9...

...Anti-CASPR/Neurexin IV [K65A/30R] CASPR/Neurexin IV Rat Mouse IgG2a 219449 Anti-CASPR/Neurexin IV [K65A/...tissue culture cells (e.g., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media...IgG2a 114507 Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R] Neuregulin-HBD (Heparin ...Pan-SAPAP Rat Mouse IgG2a 114530 Anti-Neurexin-1-Beta [N170A/1.1R] Neurexin-1-Beta Human Mouse IgG2a 114533 ...Rat Mouse IgG2a 128625 Anti-CASPR/Neurexin IV [K66/38R] CASPR/Neurexin IV Rat Mouse IgG2a 128626 Anti-Nav1.1...Rat Mouse IgG2a 177445 Anti-CASPR/Neurexin IV [K65/35R] CASPR/Neurexin IV Rat Mouse IgG2a 177446 Anti-CASPR2...IgG2a 182103 Anti-Neuregulin-CRD (Cysteine-rich domain, Type III) [N126B/31R] Neuregulin-CRD (Cysteine-rich...

...your protein is targeted to the same structure. General Structures Eukaryotic cells contain a variety of...of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent ... membrane trafficking pathways and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent ...localization with markers for common cellular structures. In other cases, your experiment might rely on... on visualizing particular cellular structures or organelles, to observe cytoskeleton remodeling or blocked...targeted to various organelles or subcellular structures. To compare with a well-characterized marker'... of organelles and subcellular structures, each with their own unique function. The nucleus, for example...

...different optimal editing windows. Be sure to refer to the literature associated with your chosen editor....activity. Be sure to sequence the genomic region you intend to target in your samples to ensure there are...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different...(Cas enzyme and guide RNA) for your experiment (Figure 1). You will decide how to express Cas9, the delivery... and finally how to validate your genetic edit. Figure 1: Flow chart describing the general framework ...and essentially function as a “knockout,” but be sure to consider potential consequences of having a non-functional...only compatible with smaller Cas enzymes, like S. aureus Cas9. For more information, see our blog post on...

...Nishimasu H, Nureki O, Zhang F. Nature . 2015 Jan 29;517(7536):583-8. doi: 10.1038/nature14136. Epub 2014... Sharp PA, Zhang F. Nature . 2015 Apr 9;520(7546):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. PubMed...Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature Protocols 2013). For cloning information, please... trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system...be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized...single vector system uses a smaller Cas9 from S. aureus (SaCas9) that has been human codon-optimized, and...guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes ...

...fluorescence to reach half its maximal value. Structure describes the oligomeric state of the protein.... (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization... (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization... (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids AcGFP1 475 505 28 Prone to dimerization... (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP 513 527 51 6.9 Prone to dimerization... (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5... (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 h Monomer...

... are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease...useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large number...single nucleotide the TAL effector will recognize: ( Figure 1B ) HD targets cytosine, NI targets adenenine,...is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove...-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is the ease with which...into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove group also hosts web-based software...The site, which will be updated soon with more features, is already receiving hundreds of unique visitors...

...human fibroblasts to neurons. Nature. 2011 Jul 13. doi: 10.1038/nature10323. Crabtree Fibroblasts Motor ... Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency. Cell. 2015 Jul 16;162(...Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem...cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):...cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac... fibroblasts to induced pluripotent stem cells. Nature. 2009 Apr 9;458(7239):766-70. Nagy Lentivirus Mouse...cardiomyocytes using calcium activity as a functional measure of success. Mol Cell Cardiol. 2013 Apr 13;60C:97...

...preparations are added to cells in culture. Three to five days later, cell cultures are inspected for sterility...droplet digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing and standardizing...quantity of gram-negative bacterial endotoxin is ensured to be less than 5 endotoxin units per mL. The endotoxin...used to determine purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre...staining and appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation...packaging system developed by the Trono lab . Cell culture medium (OptiPro SFM) containing lentivirus is first...

...(dark) acceptor, used for FLIM measurements Note that FRET measurements can be influenced by many factors...plasmids and resources for FRET experiments to measure protein interactions or conformation changes. Plasmid...use in FRET experiments. FRET efficiency can be measured using fluorescence microscopy or spectroscopy ... and emission of a photon) of the donor can be measured using fluorescence lifetime imaging microscopy...environmental factors. When designing your experiment, be sure to refer to published reports and include appropriate...Standards The articles or collections listed below feature plasmids with positive and negative FRET controls...mTurquoise2-mVenus(L68V) Goedhart et al. (2012) Structure-guided evolution of cyan fluorescent proteins ...

...which are shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each...Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi: 10.1038/nprot...described by Hochschild and colleagues ( Dove et al., Nature 1997 ) and later modified by Pabo and colleagues... & Yeh et al., PLoS ONE 2009 , Townsend et al., Nature 2009 , Zou et al., Cell Stem Cell 2009 ). Resources... practicing OPEN is available in the 2009 Joung Nature Protocols paper . How to Cite this Kit These plasmids...include Addgene in the Materials and Methods of your future publications. For your Materials and Methods section...

...Reagents References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine to tRNA^Sep with ...phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 phosphosite library configuration... reactions (SERIOHL-KILR, Barber et al., 2018). Figure 3: The library of ~110,000 phosphosites is subdivided... phosphoserine resource for phosphoproteomics . Nature Methods , 19 (11), 1371–1375. https://doi.org/10.1038...identification of phosphorylation-dependent interactions . Nature Biotechnology , 36 (7), 638–644. https://doi.org... permits programmable protein phosphorylation . Nature Communications , 6 (1). https://doi.org/10.1038...phosphoproteins using cell-free protein synthesis . Nature Communications , 6 (1). https://doi.org/10.1038...

...Glypicans and Integrins), components in the synapse (Neurexins and Neuroligins), and common epitope tags, as ...identify and interrogate with conventional reagents. Figure 1: Axon guidance cues and receptors regulate the...Cleft IPI’s Synaptic Cleft Antibody Collection features highly selective recombinant monoclonal antibodies... The initial collection includes antibodies to Neurexin and Neuroligin (Link opens in a new window) receptor...cleft proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand pairs...at neuronal synapses. Image credit: IPI Browse Neurexin and Neuroligin Antibodies ID Recombinant Antibody..., blocking ligand binding and receptor function Figure 3: IPI Integrin antibodies exhibit distinct binding...

...demonstrates enhanced transduction of cells in culture for ex-vivo conditioning. The AAV2(trpYF) serotype...AAV2(Y444F) When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava...AAV2(trpYF) When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava...4pMut)dHS When using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and...T-V) When using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava...27308302 AAV44.9 When using the AAV44.9 serotype in future publications, please acknowledge John Chiorini ...E531D) When using the AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and...

...bacteria, are also being used for their distinct features. Addgene distributes many plasmids that can help...! Protein Purification To study the function, structure, and activity of a protein it is generally necessary...yields. Proteins can then be extracted from the culture and purified through affinity chromatography. To...The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification tags compatible...generation of FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is... by a variety of small molecules, light, and temperature, in different bacterial systems. ID Plasmid Promoter...Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose Escherichia...

...SynBio Blog Genome Engineering A distinguishing feature of synthetic biology is the ease with which parts...Enzymes: SpeI , NotI , PstI Scar: TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding Prefix...Suffix Enzymes: Nhel , NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for in-frame...CTCGAG Suffix Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix start...Suffix Enzymes: SpeI , NotI , PstI Scar: ACTAGA Features: Modified RFC 10 with shortened prefix/suffix ...Enzymes: Agel , Spel , Notl, Pstl Scar: ACCGGC Features: Modified RFC 10 w/ start and stop codons; Creates...

...target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions...expanded to incorporate VCre in order to enable three-feature cell targeting. C) Example of viral targeting of...viruses delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic ...Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window) with general principles...Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PubMed...fear-promoting circuit in the extended amygdala. Nature 537(7618):97-101. PubMed (Link opens in a new window...2016. Midbrain circuits for defensive behaviour. Nature 534(7606):206-12. PubMed (Link opens in a new window...

...reprogramming the genetic code . Nature, 550 , 53–60. https://doi.org/10.1038/nature24031 (Link opens in a new window...required: The ncAA is generally supplied in the culture media. A codon must be reassigned to the ncAA. ...that is recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase and target plasmids in genome...amino acids within a protein, any alterations in structure or function in the protein can be observed. The...working off of a previously established protocol, make sure to match the growth medium, ncAA concentration, ...tRNA. Therefore many controls must be used to make sure that these conditions are true. Always express first...protein with and without the ncAA in the media to make sure that the full length protein is only made when the...

...tetracycline and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool ...synthetic Tet Response Element (TRE) typically features seven copies of tet O upstream of a minimal CMV...or skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural...even though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger expression...vector design, but it is easier to implement and ensures that all transfected or transduced cells express...can be found in Krueger et al., 2004. Some cell culture sera may contain tetracycline or its derivatives...can result in unintended induction. Check the manufacturer’s details to know whether your serum is tetracycline-free...

...interest in plants. You can search the table for features you are looking for, such as the promoter or cloning... Orzaez 1000000135 MoClo Plant Parts II and Infrastructure Kit Modules connect MoClo and Gateway for hierarchical... a selection of popular reporter plasmids that feature fluorescent, luminescent, and color-producing proteins...responsible innovation for improvement of sustainable agriculture and conservation. BAR The Bio-Analytic Resource...publications are created. Papers from the plant biology literature are discussed with one of the authors. The podcast... new window) — Collection of illustrations and pictures of plants, roots, shoots, and inflorescences -...

...are no effective therapies to prevent, treat, or cure most of these disorders. An integrated study of ...people over the age of 60. There is presently no cure or way to prevent progression of PD. Treatment consists...the idiopathic (having no specific known cause) nature of the disease, a small portion of cases can be...C9ORF72, LRRK2, Synuclein, and more. Study ER structure and function with siRNA-resistance SigmaR1 constructs...nervous system, vesicular brain cells and more. Measure synaptic transmission with improved glutamate indicators... new window) A foundation dedicated to finding a cure for Parkinson's disease through an aggressively ...

... the orientation of each target site (see Figure 1). Figure 1: Recombination outcomes are determined by...site (called loxP for Cre, or FRT for Flp). The nature of the DNA rearrangement after recombination (inversion...systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT...recombinases. Many of our in-stock viral preps feature Cre-dependent tools, or consider our AAV Packaged...recombinases. Many of our in-stock viral preps feature Flp-dependent tools, or consider our AAV Packaged...

...curated literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information...2008 to drive the development of treatments and cures for Rett Syndrome and related MECP2 disorders. The...delay between 6 and 18 months of age. A defining feature, with onset typically between 18 to 30 months, ...InterRett - international online database of clinical features and genetic characteristics of Rett syndrome (...Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol . 68, 944–950. PMID: 21154482 Neul...

...MGC129975, UI, UROC UCN2 urocortin 2 SRP, UCN-II, UCNI, UR, URP UCN3 urocortin 3 (stresscopin) MGC119002, SCP...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor A/guanylate ...cyclase A (atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide...NR61, RTR NRG1 neuregulin 1 ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, NDF, SMDF NRG2 neuregulin 2 Don-1, HRG2...HRG2, NTAK NRG3 neuregulin 3 HRG3, pro-NRG3 NRG4 neuregulin 4 DKFZp779N0541, DKFZp779N1944, HRG4 NRP1 neuropilin...response is triggered within the first few minutes of exposure to a pathogen and is the dominant defense strategy...

...with Coomassie blue. Protein band intensities are measured using ImageJ software and a standard curve is ...intensities of both the antibody and impurities are measured and the ratio of the antibody protein content ...antibody or the hybridoma-derived equivalent to ensure that potency and specificity have been retained...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

...endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP ...fluorescently tagged proteins of key cellular structures in human cell lines. Depositor...fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full...

...amplification, and high-quality quality control measures such as titering, purity assessment, sterility... comprehensive quality control processes to make sure it is ready for your experiments. Shipping 2–7 days...globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging...including purification, titration, and quality control measures. We have distributed preps around the globe and...

...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction... enables the evaluation of TALEN activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate...systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction sites for...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

...are primarily used for determining protein 3D structure and crystallography.... that aims to determine the three dimensional structures of proteins of medical relevance, and place them...empty backbones/controls) ID Plasmid GenBank Key Features 26092 p15TV-L EF456736 Hexahistidine tag with ...

...Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are up-to-...below by function. Addgene's CRISPR collections feature plasmids for a variety of organisms, including ...fused to an epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type...

...order. Experimental Disclaimer Due to the novel nature of experiments carried out with our materials, ...function of any viral vector. Addgene performs QC measures which verify the identity of the viral vector,...thorough shipping documents and following import procedure, it is the responsibility of the importer to pay...

...biology resources available at Addgene. Browse featured collections or depositing labs....Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters...papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource Synthetic...

... into mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected...Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies or ScFvs. ...

...use light produced from an enzymatic reaction to measure subtle expression changes with high sensitivity...window) is an energy transfer technique designed to measure molecular proximity in living cells. NanoBRET PPI...protein. The apparent affinity of test compounds is measured by competitive displacement of a NanoBRET tracer...

...These mutations can be inherited or arise through exposure to mutagens like radiation or viruses (e.g., HPV...Thompson T, Karsenty G, Bradley A, Donehower LA. Nature. 2002 Jan 3;415(6867):45-53. PubMed PMID: 11780111...Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly K. Nature. 1997 Jun 27;89(7):1175-84. PubMed PMID: 9215639...

...gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols, 2016 . Yamamoto ...very rapidly after the addition of auxin to the culture medium. Plasmids can be found associated with the...

...plasmid and the effects on gene expression can be measured by the luminescence output. The most popular types...substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase is secreted and...altered emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...

...-expressing BirA biotin ligase, as seen in the CAPTURE system . The locus is subsequently isolated by ...associated with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using...

...found in bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared...many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created ...

... Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection...

...regulation and developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...

...2020 research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...is embedded in an integrated knowledgebase that features, among others, genetic variants and disease associations...

...for ammonium uptake. Nature, 446 (7132):195-8. https://doi.org/10.1038/nature05579 (Link opens in a new...

...shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference....silence: conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link opens in ...

... change. Adenine DNA deaminases do not exist in nature, but have been created by directed evolution of...to activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort...