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Showing: 161 - 180 of 388 results

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR... taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...

  6. CRISPR Plasmids - Plants

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...

  7. Lentivirus Plasmids

    Type
    Collection
    ...Biosafety Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging...

  8. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...below. When targeting other gene loci, you will prepare the gene-specific CRISPR and donor vectors as well...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...removes, not just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. ...LY6C1) Stanton et al., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Resources on Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...intracellular transport machinery. Addgene offers viral preparations of AAV2-retro vectors with a variety of transgene...

  12. Sequencing Primers

    Type
    Guide
    ...Qiagen) 5' of MCS in pQE vectors, forward primer pREP Forward GCTCGATACAATAAACGCC (Invitrogen) Rous sarcoma...

  13. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Link opens in a new window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers...genetic access to cortical cell types. bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 10.1101/...

  14. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...cloning, and titering and testing your viral preparations. ...Protocols for titering and testing your virus preparations. Antibodies Protocols for common antibody applications... Video! Inoculating a Liquid Bacterial Culture Prepare and grow bacteria in liquid medium Watch the Video...long term use Watch the Video! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA...Ultracentrifugation Purify adeno-associated virus from a preparation produced in mammalian cells Watch the Video! ...physical titer of your adeno-associated virus preparation Watch the Video! AAV Titration by ddPCR Use ddPCR...physical titer of your adeno-associated virus preparation Lentivirus Titration by ddPCR Use ddPCR to measure...

  15. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration...glycerol stock before you begin your plasmid mini-prep. Try not to freeze/thaw your glycerol stock too ...15-25% glycerol. You can prepare the glycerol stock the same time you prepare your plasmid DNA. In the...

  16. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... do a mini-prep you will usually want to start 2 mL in a falcon tube, but for larger preps you might want...inoculate bacteria in liquid culture. Protocol Prepare liquid LB. For example, to make 400 mL of LB, weigh...

  17. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...the prep with full (genome containing) particles. On average, iodixanol-purified AAV preparations contain...ultracentrifugation to get pure adeno-associated virus (AAV) preps for use in your research. Protocols...Ultracentrifuge T70i rotor or equivalent Reagents Opti-Prep (60% Iodixanol) QuickSeal tubes QuickSeal tube spacers...separate out contaminants from an impure AAV preparation. The 15% iodixanol step has 1M NaCl to destabilize...iodixanol solution is available under the name OptiPrep (Link opens in a new window) from Sigma-Aldrich...Centrifugal filter units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer Dissolve 5.84 g of NaCl... and 2 mL of 5 M NaCl to 43 mL PBS Procedure Preparation and loading of the iodixanol gradient: 15% iodixanol...

  18. Fluorescence Titering Assay

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge...conical tubes, VWR 21008-216 Lentivirus preparation Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM...Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker. Protocols...This protocol can be used to titer lentiviral preparations that carry a fluorescence marker. Determining... 75,000 cells into each well of a 6-well dish. Prepare a batch of cells as follows: Dilute 525,000 cells...thaw the lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete ...

  20. Colony Formation Titering Assay

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge...conical tubes, VWR 21008-216 Lentivirus preparation Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM...This protocol can be used to titer lentiviral preparations that confer antibiotic resistance. Protocols...This protocol can be used to titer lentivirus preparations that confer antibiotic resistance. Determining...Determining the titer of your lentiviral preparation allows you to control the multiplicity of infection (MOI... Use this dose for the colony formation assay. Prepare a batch of DMEM complete containing 10 μg/mL polybrene...thaw the lentiviral aliquot on ice prior to use. Prepare 10-fold serial dilutions of the lentivirus into...
Showing: 161 - 180 of 388 results