...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...mutation than restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly...

...BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of standard, blank, and lysate samples... nm . Calculate the average absorbance of the duplicate samples on the plate. Subtract the average absorbance...

...sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and ...and small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 ...:7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect...sensor for cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) ...:4003-4012.e6. Xavier Nicol cyclic di-GMP FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical...Fluorescent biosensor of the cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with...protein for NADH/NAD+ ratio Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence...

...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications...

...Selectable Marker PI Publication Bacteria Plasmid Gene/Insert Promoter PI Publication Drosophila Plasmid... Marker PI Publication Plant Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans ... Marker PI Publication Yeast Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish Plasmid...Marker PI Publication Xenopus Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...each of the above serotypes and buffers. Please click on the plasmid name for ordering information. ID...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

...Resources Web References PAM Sequences Glossary Publications CRISPR Overview Class 2 C lustered R egularly... every genomic target. Due to its comparative simplicity and adaptability, CRISPR has rapidly become the...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR...-mediated DSB repair has important practical implications, because a population of cells expressing Cas9...requiring longer homology arms. Depending on the application, the repair template may be a single-stranded...and are thus well suited to directed evolution applications. Examples of these base editing systems include...be carried out by Cas9. Such multiplex CRISPR applications include: The use of dual nickases to generate...

... the original publication below. You can also download the full-text of the publication here . Article...bands from biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with... of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs details...single-site small indels given the efficiency of biallelic modification, ease of rapid identification by ... sgRNA located within exons will enrich even monoallelic deletion clones for loss of function. This is...useful to characterize the non-deleted allele in monoallelic deletion clones. For small deletions (as the ...is critical that the deletion and non-deletion amplicons be easily resolved on an agarose gel for multiplexing...

...efficiency than NHEJ. Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...opposite strand) mutations. Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...RT template. Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...headings. Click on the publication link to view all other plasmids from that article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

...mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...library page. Find Pooled Libraries of Interest Click on different properties to create a custom filtered...Activation Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini...Knockout Human Olzmann 3rd ~10 13,920 Human lncRNA Splicing-targeting CRISPR Library 119977 Knockout Human...Knockout Mouse Yusa 3rd 5 90,230 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy...Knockout Mouse X.S. Liu 3rd 5 5 10 49,252 Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation Human... Human Birsoy 3rd Varies 32,460 Mouse Metabolic Gene CRISPRa sgRNA Library 187081 Activation Mouse Birsoy...

...Selectable Marker PI Publication Bacteria Plasmid Gene/Insert Promoter PI Publication Drosophila Plasmid...Promoter PI Publication Plant Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans ...Promoter PI Publication Yeast Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that... PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve the tool...various species, as well as the development of new applications. The first CRISPR papers described two main ...RNA, making it a very flexible system. Other Applications Scientists have also used the targeting capability...Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease, with the...

...Marker PI Publication Bacteria Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant Plasmid... Marker PI Publication Yeast Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...and are thus well suited to directed evolution applications. Examples of these base editing systems include...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis...survival and proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene...Sabatini . Return to top mTOR Pathway - Gene List Click on a name to find available plasmids for the gene...

...control). The end result of the meeting will be a publicly shared practical guide for all scientists in the...useful to help improve this technology or its application? What are some current obstacles that researchers...prepare and compile resources for this meeting's publication, we are asking participants to share practical...antibodies and recommended dilutions, etc.), novel applications, or any other useful information. Experimental...have final approval before anything is released publicly, but please only share materials that you are ...materials that you have permission to share (e.g., publication's permission). Connie Cepko, Harvard Medical School...section for how to best help contribute to this publication. Citation information for openly shared communication...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...