...nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created... of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA ...modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions... Cas9 is fused to activation-induced cytidine deaminase (AID). Want more information on the wide variety...

... and H 2 O to final volume of 50 μl. This reaction may be scaled down to a smaller final volume if necessary...video, you can find the protocol section from the original publication below. You can also download the full-text...., sgRNA-B in Table 4 ) and do not add C to the final position of the reverse complement oligo. In this...Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos...contains 50 μl cell culture media per well for a final volume of 150 μl. This facilitates screening by ...research. Gehl J. Acta Physiologica Scandinavica . 2003. PubMed . Purifying plasmid DNA from ...

... 5, rg*, PHP.eB Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV....NLS-mRuby2 Constitutive 1, 2, 8, 9, rg*, PHP.eB Gradinaru 100896 pAAV.GFA104.PI.eGFP.WPRE.bGH GFA104 EGFP...hSyn eYFP Constitutive 2, 5, 8, 9, rg*, PHP.eB Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet ...pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre...pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Gradinaru 137131 pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent...

...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

...innovation for improvement of sustainable agriculture and conservation. Medicinal Plant Genomics Resource (...other monocots - Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic genome...in a new window) - The initiative promotes interdisciplinary exchange, open technologies, and responsible...Transcriptomic and metabolomic resources for 14 key medicinal plants for the advancement of drug production ...

... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific base editor that can...MS2 hairpin-tagged gRNA to recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window...the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable...Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...Marker PI Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP...

...sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Reference genomes include Arabidopsis...Proof-of-concept performed in zebrafish. ZiFiT Targeter Originally developed to identify zinc finger nuclease sites...and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative...pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF 102.5 KB Jaenisch CRISPR plasmid summary... , pAC1 , pAC147 , pAC148 , pAC149 ; gateway destination: pAC90 PDF 1.0 MB Katic Nematode: Cas9 and gRNA...

...CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina Schroer...17800 pM-ErbB4-delta-kinase ERBB4 GAL4-BD ALS Marius Sudol 17801 pM-ErbB4-delta-kinase-PY1 mutant ERBB4 GAL4... GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD ALS Marius Sudol 17803 ... CMV Parkinson's Mark Cookson 25071 2XMyc-LRRK2-Kinase LRRK2 Myc CMV Parkinson's Mark Cookson 25072 2XMyc-LRRK2...ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1...ERBB4 CMV ALS Yardena Samuels 29544 pCDF1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29591 426Gal-FUS...type IE Arthur Riggs 37057 GFP-SMN SMN1 GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1...

... Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-...Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou...Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley ...Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

... Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors...synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains...Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource ...

...18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries 92385 Human CRISPR inhibition...Celltag library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal...

...Mb-Fluoro-Phe B5 Pyl Fluoro-Phe B5 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...Mb-Fluoro-Phe D6 Pyl Fluoro-Phe D6 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...sites changes to UAG, RF1 function removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church...codon, and tyrU gene replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses...tyrTV and tyrU genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses...

...factors and the sensor behavior may differ from the original characterization (e.g. in vitro vs. in vivo , ...Zhou Calcium Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids...biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7....charges in live cells. Nat Biotechnol. 2017 Mar 13. Katharina Gaus Endo/Exocytosis Superecliptic pHluorin fused...-pHluorin Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal ...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy...

...Find recombinant antibodies targeting epitope tags and common fusion proteins....common cellular proteins. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

...Find recombinant antibodies targeting neuroscience-related proteins....in neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

...Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection Disease Research Michael... Simpson Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project ... Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral...

...Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600... in living animals. Further development of the original iRFP has resulted in a split fluorescence complementation...

...Browse Addgene's collection of recombinant monoclonal antibodies....Addgene distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-...Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype Reactivity...

...you have the right to use the original plasmid. Distribution of the original plasmid is important because...which is a sample of the purified DNA from the original plasmid that was used to make the virus. In order...

...gamma GFP Tom Misteli 55068 mCherry-LaminA-C-18 Nuclear Envelope LaminA1 mCherry* Michael Davidson 98831 ...Davidson 98822 Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821...98821 Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1...

...in order to proceed with any adjustments to the original order. Turnaround Time Production of a Packaged...the approval of the MTA by your institution. Our final step of the QC process, viral genome sequence verification...in order to proceed with any adjustments to the original order. Experimental Disclaimer Due to the novel...

...Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics... Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications ...

...as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and titer viral...research-grade use. Many chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository...vivo testing. We recommend that you consult the original paper of your chosen AAV plasmid for more information...

...virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP or frt...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors...

...Aqua Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian...pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of...pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene ...

...Stable RNA <8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...Toth K. Adenovirus vectors for gene therapy, vaccination and cancer gene therapy. Curr Gene Ther. 2013...

...9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase I, Large...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

... found on this page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 ... Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting the SARS-... Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-...

...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine is..., Davidson AR. 2013. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...

...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate scientific research...

...assembly are different among the three methods, the final products are the same). All expression vectors listed...Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287...

...Catalytically inactive CRISPR plasmids for purification of target loci.... molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA bound by the...

...Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...

... can mediate gene replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro...51026 U6-BbsI-crRNA : Generates crRNA for use in combination with tracrRNA. 51434 pHD-DsRed : vector for generating...

...a ntiviral de fense) complex, comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined...

...partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative (IMI) of...Figure 1: Representation of RESOLUTE mission. The combination of metabolomics and genome engineering forms ...

...Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN...tools for using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove...

...institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression...experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate dehydrogenase...

...the most common of which involves homologous recombination of adenovirus plasmids in either mammalian cells...Strain Description PI 16398 BJ5183 Strain for recombination between shuttle plasmid and adenoviral plasmid...

... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...