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Showing: 1 - 50 of 17834 results

  1. pAAV-EF1a-iCreV (AAV1)

    Viral Prep
    #140135-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  2. pAAV-EF1a-iDreV (AAV1)

    Viral Prep
    #140136-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Tags
    None
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  3. pAAV-EF1a-iCreV (AAV PHP.eB)

    Viral Prep
    #140135-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-EF1a-iDreV (AAV PHP.eB)

    Viral Prep
    #140136-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Tags
    None
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  5. rAAV-CAG::FLEX-rev:: hM4D-2a-GFP (AAV1)

    Viral Prep
    #52536-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from rAAV-CAG::FLEX-rev:: hM4D-2a-GFP (#52536). In addition to the viral particles, you will also receive purified rAAV-CAG::FLEX-rev:: hM4D-2a-GFP plasmid DNA. CAG driven, Cre-dependent expression of hM4D and GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Scott Sternson
    Article
    Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:
    Promoter
    CAG
    Tags
    GFP
    Available since
    Dec. 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105635-plasmid-map-sequence-id-203205

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  9. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid
    #105637
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105637-plasmid-map-sequence-id-203819

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  10. pRSV-Rev

    Plasmid
    #12253
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    Rev (rev )
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12253-plasmid-map-sequence-id-443803

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  12. pVE2_4_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS(rev)

    Plasmid
    #154237
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS(reversed)+CP_pcbC
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Mutation
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154237-plasmid-map-sequence-id-305989

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  26. pCCG_nit9fl_Gephyrin_linker_reverse

    Plasmid
    #228417
    Purpose
    Expression of NIT-9 domains in reverse orientation with Gephyrin linkage region in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9 domains reversed (GPHN Human)
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    removal of the linkage region and insertion of th…
    Promoter
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228417-plasmid-map-sequence-id-460294

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  27. pCCG_nit9fl_CNX1_linker_reverse

    Plasmid
    #228415
    Purpose
    Expression of NIT-9 domains in reverse orientation with CNX1 linkage region in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9 domains reversed (AT5G61790 Mustard Weed)
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    removal of the linkage region and insertion of th…
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    228415-plasmid-map-sequence-id-459561

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  28. pCCG_nit9fl_reverse

    Plasmid
    #228410
    Purpose
    Expression of NIT-9 with reversed domains in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9 domains reversed
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    domains reoriented
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228410-plasmid-map-sequence-id-459450

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  31. prCOrev

    Plasmid
    #153961
    Purpose
    Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
    Use
    Retroviral; A reporter plasmid detecting dna reco…
    Tags
    NLS-ZF
    Expression
    Mammalian
    Mutation
    No mutation
    Promoter
    LTR
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153961-plasmid-map-sequence-id-306376

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  32. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126882-plasmid-map-sequence-id-249649

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  39. pSW648 - pREV>>

    Plasmid
    #115996
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Sebastien Wolf
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Use
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    115996-plasmid-map-sequence-id-242815

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  40. pCDZ2FE_Rev3_1873-2025_4PA

    Plasmid
    #105673
    Purpose
    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Rev3L (REV3L Human)
    Use
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P1880A, P1885A, P1996A, P2001A
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105673-plasmid-map-sequence-id-203204

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  41. pREVtre GFP

    Plasmid
    #128069
    Purpose
    Retroviral tet inducible EGFP
    Depositor
    Oskar Laur
    Insert
    tet inducible EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    no
    Promoter
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128069-plasmid-map-sequence-id-255185

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  42. pREVTRE-AID

    Plasmid
    #121067
    Purpose
    Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio
    Depositor
    Randy Poon
    Article
    Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    AID
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    121067-plasmid-map-sequence-id-234361

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  44. pRevTRE-FLMKL1

    Plasmid
    #27162
    Depositor
    Ron Prywes
    Insert
    MKL1 (MRTFA Human)
    Use
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 22, 2010
    Availability
    Academic Institutions and Nonprofits only

  47. pLp6.3-Reverse

    Plasmid
    #79117
    Purpose
    Lentiviral expression of EglN3-Reverse
    Depositor
    Susanne Schlisio
    Insert
    control plasmid with reverse EglN3 insert (EGLN3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    79117-plasmid-map-sequence-id-149810

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  49. p3E-rev_U6_MCS

    Plasmid
    #109769
    Purpose
    Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    U6-gRNA cassette
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109769-plasmid-map-sequence-id-224177

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Showing: 1 - 50 of 17834 results