We narrowed to 7,717 results for: nls

1. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

   Plasmid

   #64113

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

   Plasmid

   #64112

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

   Plasmid

   #69232

   Purpose

   Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

   Plasmid

   #69233

   Purpose

   R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Promoter

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

   Plasmid

   #69231

   Purpose

   Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 TALE
   

   Use

   CRISPR

   Tags

   2X NLS and NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSimpleII-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47867

   Purpose

   This plasmid encode NmCas9 with two NLS and a HA tag

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV_NLS-dSaCas9-NLS-VPR

   Plasmid

   #68495

   Purpose

   AAV vector containing nuclease null SaCas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   dSaCas9
   

   Use

   AAV

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV_NLS-SaCas9-NLS-VPR

   Plasmid

   #68496

   Purpose

   AAV vector containing SaCas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SaCas9-VPR
   

   Use

   AAV and CRISPR

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Use

   Tags

   GFP11x7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-3xFLAG-NLS-SpCas9-NLS

    Plasmid

    #51307

    Purpose

    Cas9 expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Insert

    3xFLAG-NLS-SpCas9-NLS
    

    Use

    CRISPR; Xenopus tropicalis expression

    Tags

    3x Flag and NLS

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-TO-nls-st1dCas9-3nls-3XGFP

    Plasmid

    #64111

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sox1/2/3-2373/+3>nls::Cas9::nls

    Plasmid

    #59989

    Purpose

    Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Sox1/2/3 (SoxB1) -2373/+3

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mesp-1916/-1>nls::Cas9::nls

    Plasmid

    #59988

    Purpose

    Mesp driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Mesp -1916/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47868

    Purpose

    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available Since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

    Plasmid

    #69219

    Purpose

    Expresses wild type SpCas9 fused to Zif268 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    Zif268 (Egr1 Mouse)
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

    Plasmid

    #69227

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69225

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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