Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,966 results for: puc

Clear keywords
Showing: 451 - 500 of 6966 results

  11. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    58807-plasmid-map-sequence-id-91105

    Enlarge

  14. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Available Since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    40306-plasmid-map-sequence-id-115990

    Enlarge

  16. pUC19-gRNA

    Plasmid
    #137776
    Purpose
    Contains the T7 promoter and gRNA scaffold and is used for the in vitro transcription of gRNAs
    Depositor
    Kabin Xie
    Insert
    gRNA scaffold
    Use
    CRISPR
    Mutation
    pUC19 BsaI destroyed
    Promoter
    T7
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137776-plasmid-map-sequence-id-272095

    Enlarge

  20. pUCP20 PilB

    Plasmid
    #162845
    Purpose
    Expression of PilB with a C-terminal histag
    Depositor
    Katrina Forest
    Insert
    PilB
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162845-plasmid-map-sequence-id-327693

    Enlarge

  26. pUCmEos3.2:cat

    Plasmid
    #78100
    Purpose
    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.
    Depositor
    Peng Chen
    Article
    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1
    Insert
    mEos3.2-cat
    Expression
    Bacterial
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pUC57KAN-20XHA

    Plasmid
    #233114
    Purpose
    20X HA tag multimer
    Depositor
    Steve Stowers
    Insert
    HA tag
    Use
    Unspecified
    Mutation
    None
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233114-plasmid-map-sequence-id-466707

    Enlarge

  35. pUC19-T7-SdB-T

    Plasmid
    #87307
    Purpose
    Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Stabilized dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87307-plasmid-map-sequence-id-174665

    Enlarge

  50. pUC19-E6MBW6

    Plasmid
    #103102
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103102-plasmid-map-sequence-id-209054

    Enlarge

Showing: 451 - 500 of 6966 results