We narrowed to 18,142 results for: Met;

1. pFAB1902

   Plasmid

   #47858

   Purpose

   BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   M13_central_T_linker_rrnD_T1 double terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   concatenate of M13 central and rrnD

   Promoter

   pLTetO13

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFAB3997

   Plasmid

   #47828

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 14 and BCD21
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   see insert

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFAB3912

   Plasmid

   #47815

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 14 and BCD8
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   see insert

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AB.pCCL.sin.cPPT.U6.miR-431-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46620

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-431 Decoy (MIR431, Mir431 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pPB9

   Plasmid

   #210036

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   puro-T2A-eGFP
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xCBX7CD_W35A-IFP2.0C

   Plasmid

   #215755

   Purpose

   Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2xCBX7CD_W35A-IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXS-IRES-Blast CG1358

   Plasmid

   #218541

   Purpose

   Drosophila CG1358 cDNA (human codon optimized)

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   CG1358 (HisT Fly)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-Blast SLC5A7

   Plasmid

   #218539

   Purpose

   human SLC5A7 cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SLC5A7 (SLC5A7 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXS-IRES-Blast SLC44A1

    Plasmid

    #218538

    Purpose

    human SLC44A1 cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    SLC44A1 (SLC44A1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. plentiCRISPR-v2 Puro SPNS1_sg2

    Plasmid

    #218531

    Purpose

    sgRNA targeting human SPNS1

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    SPNS1 (SPNS1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. plentiCRISPR-v2 Puro SPNS1_sg1

    Plasmid

    #218530

    Purpose

    sgRNA targeting human SPNS1

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    SPNS1 (SPNS1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKG-GW1-SRT1

    Plasmid

    #203171

    Purpose

    Yeast expression vector for yeast SRT1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    SRT1 (SRT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 2x3APWWP_K299E_D333A-IFP2.0C

    Plasmid

    #215753

    Purpose

    Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2x3APWWP_K299E_D333A-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCF702

    Plasmid

    #209650

    Purpose

    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

    Depositor

    Bastian Molitor

    Article

    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

    Inserts

    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TLNRD1_35-345_eGFP

    Plasmid

    #208881

    Purpose

    Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acid sequence 35-345

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mito-PDCD10-mScarlet-I

    Plasmid

    #208887

    Purpose

    Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM3 (PDCD10 Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    PDCD10 targeted to the mitonchondria

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TLNRD1_5H_eGFP

    Plasmid

    #208861

    Purpose

    Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 5 helix bundle (TLNRD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acid 142-273

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

    Plasmid

    #174897

    Purpose

    ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sf GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAA in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

    Plasmid

    #174898

    Purpose

    opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TGA in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-TRE-IEo

    Plasmid

    #201992

    Purpose

    AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

    Depositor

    Wei Xu

    Article

    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

    Insert

    Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Tight TRE

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKG-GW1-RER2

    Plasmid

    #203170

    Purpose

    Yeast expression vector for yeast RER2

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    RER2 (RER2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKG-GW1-GlcisPT

    Plasmid

    #203172

    Purpose

    Yeast expression vector for Giardia cis-prenyltransferase

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    UPPS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-RAPH1Δ536-587

    Plasmid

    #194865

    Purpose

    Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 536-587 deleted

    Promoter

    CMV

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFP-MYO10-FERM-ITGBD

    Plasmid

    #194855

    Purpose

    Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 S2001_F2002insA/T2009D (MYO10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    MYO10 has the following mutations S2001_F2002insA…

    Promoter

    CMV

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSFDuet-1_VNp-mNeongreen-Uricase

    Plasmid

    #182417

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-uricase
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSFDuet-1_mNeongreen-StefinA

    Plasmid

    #182418

    Purpose

    Bacterial expression of mNeongreen-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-stefin (CSTA Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNHT7::UMSBP::sNluc2_ORF1

    Plasmid

    #186765

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGAPDH::sNluc1

    Plasmid

    #187221

    Purpose

    A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Overexpression

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNHT7::ENO::sNluc2

    Plasmid

    #186770

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNHT7::RPL15::sNluc0

    Plasmid

    #186771

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNHT7::RPL15::hNluc

    Plasmid

    #186772

    Purpose

    An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNHT7::YB1::hNluc

    Plasmid

    #186774

    Purpose

    An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNHT7::YB1::sNluc1

    Plasmid

    #186754

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNHT7::ENO::sNluc1

    Plasmid

    #186755

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNHT7::RPL10::sNluc1

    Plasmid

    #186756

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNHT7::RPL15::sNluc1

    Plasmid

    #186757

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNHT7::YB1::sNluc0

    Plasmid

    #186758

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNHT7::YB1::sNluc2

    Plasmid

    #186759

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pNHT7::YB1::sNluc3

    Plasmid

    #186760

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge