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We narrowed to 15,064 results for: pET

Showing: 5001 - 5050 of 15064 results

  1. pHIT334

    Plasmid
    #204516
    Purpose
    Bacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C-terminal fragment of coh-3 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204516-plasmid-map-sequence-id-403820

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  2. pHIT186

    Plasmid
    #204513
    Purpose
    Bacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    PAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204513-plasmid-map-sequence-id-403809

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  3. pHIT521

    Plasmid
    #204520
    Purpose
    Bacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-5 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204520-plasmid-map-sequence-id-403749

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  4. pHIT182

    Plasmid
    #204511
    Purpose
    Bacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a with D769A mutation (C. elegans) (csr-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    D769A
    Promoter
    T7lac
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204511-plasmid-map-sequence-id-403747

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  5. pHIT520

    Plasmid
    #204519
    Purpose
    Bacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204519-plasmid-map-sequence-id-403744

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  6. pHIT342

    Plasmid
    #204514
    Purpose
    Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of syp-1 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204514-plasmid-map-sequence-id-403743

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  7. pHIT320

    Plasmid
    #204517
    Purpose
    Bacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    Internal fragment of him-1 (C. elegans) (him-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204517-plasmid-map-sequence-id-403742

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  8. pSLS.539

    Plasmid
    #184958
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fabH donor G954A
    Promoter
    T7/lac
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    184958-plasmid-map-sequence-id-381448

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  10. pSLS.543

    Plasmid
    #184960
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A
    Promoter
    T7/lac
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184960-plasmid-map-sequence-id-376545

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  11. pSLS.545

    Plasmid
    #184962
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    priB donor G1069A
    Promoter
    T7/lac
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184962-plasmid-map-sequence-id-376122

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  13. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86972-plasmid-map-sequence-id-173929

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  25. pKSJ376

    Plasmid
    #103065
    Purpose
    Expresses colicin E1 with R domain deletion and ImmE1
    Depositor
    Alan Finkelstein, Karen Jakes
    Article
    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0
    Inserts
    colicin E1 immunity protein (pColE1_26, imm E. coli)
    cea/colicin E1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    R (receptor-binding domain) deletion in colicin E…
    Promoter
    T7 lac (from pET) and T7 lac (from pET52-b)
    Available Since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    103065-plasmid-map-sequence-id-200637

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  28. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

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  35. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Article
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Use
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available Since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

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  41. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  42. pR1G108CP20X

    Plasmid
    #85086
    Purpose
    Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1
    Promoter
    T7 promoter
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    85086-plasmid-map-sequence-id-164463

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  46. pEMI91

    Plasmid
    #74888
    Purpose
    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information
    Depositor
    Piotr Marszalek
    Article
    Scholl et al Biomacromolecules. 2016 Jun 8.
    Insert
    Titin I27 x 9 (TTN Human)
    Use
    Tags
    His-tag and Strep-tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74888-plasmid-map-sequence-id-141569

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  49. pMSP1D1R1

    Plasmid
    #97323
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1R1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of R residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97323-plasmid-map-sequence-id-182662

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  50. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

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Showing: 5001 - 5050 of 15064 results