Addgene

We narrowed to 33,671 results for: CaS

Showing: 2001 - 2020 of 33671 results

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pST1374-GCN4-dCas9

Plasmid

#113023

Purpose

Expresses GCN4-dCas9 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

GCN4-dCas9 (GCN4 S. pyogenes, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

D10A, D839A, H840A, N863A

Promoter

CMV

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rtTA and TagBFP cassettes

Plasmid

#62452

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas6

Plasmid

#126485

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR2-HA Cterm

Plasmid

#84489

Purpose

overexpression of CASTOR2 with HA tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pX551-miniCMV-CjCas9

Plasmid

#107033

Purpose

AAV plasmid expressing CjCas9 under control of miniCMV promoter

Depositor

Alex Hewitt

Insert

CjCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113342

Purpose

Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 Exons 1-3

Plasmid

#91799

Purpose

Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

Laccase2 (stw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-AkCas12b-2AeGFP

Plasmid

#121946

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pX551-miniCMV-SaCas9

Plasmid

#107032

Purpose

AAV plasmid expressing SaCas9 under control of miniCMV promoter

Depositor

Alex Hewitt

Insert

SaCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-ACTG1

Plasmid

#66943

Purpose

CRISPaint target selector ACTG1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA ACTG1

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-indRecA56

Plasmid

#102294

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Use

CRISPR

Tags

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-Gsk3b(new)

Plasmid

#122341

Purpose

Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Gsk3b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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RCAS DNhRARalpha (CC#1865)

Plasmid

#15153

Depositor

Connie Cepko

Article

Sen et al Development. 2005 Dec . 132(23):5147-59.

Insert

RAR alpha (dom neg) (RARA Human)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Dominant negative. Truncated at aa403, so lacks t…

Promoter

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

Plasmid

#112921

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAC1803_pmax-RBM38-dCasRx

Plasmid

#118637

Purpose

Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-dCasRx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEGB 35s:dCas:BRD:tNos (GB1172)

Plasmid

#75401

Purpose

Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

dCas9:BRD

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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