We narrowed to 12,166 results for: ena
-
Plasmid#127237PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
SP-GFP-CD274-UTR
Plasmid#121142PurposeMammalian expression of GFP tagged CD274 with its own 3'UTRDepositorInsertCD274 with its own 3'UTR (CD274 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rssA
Plasmid#89957PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.DepositorInsertrssA gRNA
ExpressionBacterialPromoterpTetAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10E
Plasmid#91209PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: noneDepositorInsert35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP
Plasmid#185686PurposeLow-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.DepositorInsertERmembrane-GST-HaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-RIPK3
Plasmid#78829Purposeretroviral expression of RIPK3DepositorAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1183
Plasmid#119254Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-V5_pRS415
Plasmid#199667Purposeexpresses LOV-Turbo in the yeast cytosolDepositorInsertLOV-Turbo
TagsV5ExpressionYeastPromoterGal1,10Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6sgp53-U6sgbbSapI-GFAPCre
Plasmid#100275PurposeAAV-CRISPR mediated genome editing in glia / astrocytesDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…ExpressionMammalianAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS4
Plasmid#60785PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS4
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a
Plasmid#205304PurposeExpression of WHaloCaMP1a in E. coliDepositorInsertWHaloCaMP1a
ExpressionBacterialAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-hBACCS2
Plasmid#67626PurposeMammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switchDepositorInsertORAI1-hBACCS2
TagsHAExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-SYP-HRP
Plasmid#117186PurposePlasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertSYP-HRP
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDB_156
Plasmid#216090PurposeCas9 [Sp] CRISPRa targeting CD274, positive controlDepositorInsertCD274 guide
UseCRISPR and Lentiviral; Positive controlsAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Venus-pLenti6
Plasmid#36948DepositorInsertVenus (EYFP)
UseLentiviralTagsFLAGExpressionMammalianMutation-F46L/F64L/V68L/M153T/V163A/S175GPromoterCMVAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG-FFLuc-3XFLAG
Plasmid#127333PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codonDepositorInsertFirefly luciferase
Tags3xFLAGExpressionMammalianPromoterCV40Available SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB1F+6
Plasmid#190835PurposeFor mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJRH623
Plasmid#171633Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-non-targeting
mNeon
UseCRISPR and LentiviralExpressionMammalianPromoterEF1-a and U6Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα- T391A_WPRE
Plasmid#196676PurposeAAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).DepositorInserttAKARα-T391A
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4(RVH)-Bre1
Plasmid#116885PurposeFLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutationsDepositorInsertLINXa4(RVH)-Bre1
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11A, LINXa3 wi…PromoterADH1Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrc99a-NoSS-TolC
Plasmid#155179PurposeExpresses signal sequence-less TolC as inclusion bodiesDepositorInsertTolC (tolC )
ExpressionBacterialMutationdeleted residues 1-20, residue 21 mutated to Met …PromotertrcAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSP3-FR-2Strep
Plasmid#210315PurposeExpresses SARS-CoV-2 NSP3 in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1185
Plasmid#119255Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGFAP-Pink Flamindo-WPRE-SV40
Plasmid#178790PurposeAAV vector to express the red cAMP indicator in astrocytesDepositorInsertPink Flamindo
UseAAVPromoterhGFAPAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4(RLR)-Bre1
Plasmid#116884PurposeFLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutationsDepositorInsertLINXa4(RLR)-Bre1
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11A, LINXa3 wi…PromoterADH1Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9-MS2-BB_BbsI
Plasmid#164802PurposeREDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFPDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21A
Plasmid#91128PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-HA-mNG21-10
Plasmid#141213PurposeMembrane Tethered Split Fluorescent Reporter for ER RetragradeDepositorInsertSec61b-HA-mNG21-10
UseLentiviralTagsHAExpressionMammalianAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)GFP-9xGGS-Cre-6xHis
Plasmid#62372PurposeExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cellsDepositorInsert(-30)GFP-9xGGS-Cre-6xHis
Tags(-30)GFP and 6xHis tagExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger3-TS-EYFP
Plasmid#127240PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAT007
Plasmid#171634PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-TRAC
mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterCAG and U6Available SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-nSyb-IVS-PhiC31
Plasmid#133868PurposeReplace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.DepositorInsertPhiC31 Recombinase (int )
ExpressionInsectAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
Plasmid#83947PurposegRNA cassette-carrying vector with natMX markerDepositorInsertgRNA targeting ADE2 gene
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDB_118
Plasmid#216089PurposeCas9 [Sp] CRISPRa targeting CD47, positive controlDepositorInsertCD47 guide
UseCRISPR and Lentiviral; Positive controlsAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…Available SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-3HA
Plasmid#67616PurposeA pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…Tagsthree inframe 3' HA tagsExpressionMammalianPromoterCMV and SP6Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only