Addgene

We narrowed to 10,181 results for: AGA

Showing: 3151 - 3180 of 10181 results

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PL-SIN-EF1α-EGFP

Plasmid

#21320

Purpose

Control Lentiviral vector with strong EF1a promoter, expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-Hs beta1

Plasmid

#45927

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Hs Beta1 adaptin (AP1B1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pLM-fSV2A

Plasmid

#27512

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

OCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

Plasmid

#87693

Purpose

Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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457 p3E-2A-EGFPpA

Plasmid

#195967

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEE391

Plasmid

#149281

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_FT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-empty

Plasmid

#105594

Purpose

empty control vector with GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

no insert, empty control vector

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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#1.4 pre-G-SynapShot-TagBFP

Plasmid

#214717

Purpose

Expresses green #1.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 pre-G-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-cyto-Ruby3-iATPSnFR1.0

Plasmid

#102554

Purpose

expresses cytoplasmic ATP sensor in astrocytes with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-NLS-PhoCl-mCherry

Plasmid

#87691

Purpose

Express NLS-PhoCl-mCherry in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NLS-PhoCl-mCherry

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBJ7

Plasmid

#167136

Purpose

A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Kanamycin promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBJ1

Plasmid

#167130

Purpose

A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Frr promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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763 pME mApple

Plasmid

#195960

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Use

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-C(3+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Article

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAd-Klf4

Plasmid

#19770

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Science. 2008 Sep 25. ():.

Insert

Klf4 (Klf4 Mouse)

Use

Adenoviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pBJ6

Plasmid

#167135

Purpose

A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Kanamycin promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBJ5

Plasmid

#167134

Purpose

A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Frr promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA_Tet3

Plasmid

#85740

Purpose

shRNA against mouse Tet3

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

Tet3 (Tet3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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neurod1:CaMPARI

Plasmid

#173479

Purpose

CaMPARI is driven by -5kb neurod1 promoter.

Depositor

John Rawls

Article

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

CaMPARI

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6B

Plasmid

#42022

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6B (TNRC6B Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-CAR-GECO1

Plasmid

#45493

Purpose

Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

CAR-GECO1

Use

Tags

Expression

Mammalian

Mutation

R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDisplay sHRPb-KDEL

Plasmid

#73150

Purpose

Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-KDEL

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEE392

Plasmid

#149282

Purpose

T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_mFT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
460 p3E-2A-mCherrypA

Plasmid

#195970

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-convex

Plasmid

#132696

Purpose

pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_convex including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCX-ThPDE5VV

Plasmid

#134778

Purpose

cGMP FRET biosensor

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

ThPDE5VV

Use

Tags

cpVenus-Venus and mTurquoise2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-O-GECO1

Plasmid

#46025

Purpose

Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

O-GECO1

Use

Tags

Expression

Mammalian

Mutation

R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

Promoter

CMV

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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