We narrowed to 15,989 results for: LEA

1. pDuBir-Lbu-dCas13a-avitag

   Plasmid

   #100817

   Purpose

   Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

   Depositor

   Mitchell O'Connell

   Article

   Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

   Insert

   Lbu_Cas13a_R472A_H477A_R1048A_H1053A
   

   Tags

   AviTag and His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   T7

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4396

   Plasmid

   #74041

   Purpose

   Expresses human codon-optimized AsCpf1 and As crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   As crRNA
   AsCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV and human U6

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGT9050

   Plasmid

   #219429

   Purpose

   PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   AATG_PapE-4xLF2_AGGT
   

   Use

   Moclo compatible level 0 module

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pExpreS2-1-C3-PA-10H-SIII

   Plasmid

   #175443

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tmem38A pEGFP-N2 (1401)

   Plasmid

   #62050

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem38A (TMEM38A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Tmem38A mRFP-N2 (800)

   Plasmid

   #62007

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem38A (TMEM38A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMSCV-BirA-P2A-mCherry

   Plasmid

   #74056

   Purpose

   retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   BirA biotin ligase
   

   Use

   Retroviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   MSCV-LTRs

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNLS-2xBFP(4C)

   Plasmid

   #176151

   Purpose

   Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   NLS-2xBFP(4C)
   

   Tags

   6xHis tag and SV40 nuclear localization sequence …

   Expression

   Bacterial

   Mutation

   Compared with EGFP, the two BFP domains have the …

   Promoter

   Ptrc

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

   Plasmid

   #134864

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   emerin (EMD Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Akt-STOPS-NLS

    Plasmid

    #175007

    Purpose

    Nuclear-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExodus CMV.Trex2

    Plasmid

    #40210

    Purpose

    Mammalian expression of codon-optimized Trex2.

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

    Insert

    Three prime repair exonuclease 2 (Trex2 Mouse)
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. SWEET11:SWEET11-2A-GFP-GUS

    Plasmid

    #172484

    Purpose

    Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

    Insert

    SWEET11 (AT3G48740 Mustard Weed)
    

    Tags

    GFP, GUS

    Expression

    Plant

    Promoter

    SWEET11

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-Hsp90 M(272-617)

    Plasmid

    #22482

    Purpose

    Please note this plasmid contains GST-Hsp90 residues 273-617.

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90alpha M domain (HSP90AA2P Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-Rheb(C181S)

    Plasmid

    #192437

    Purpose

    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb(C181S) (RHEB Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Rheb Cys 181 mutated to Ser; abolishes C-terminal…

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUW-TetO-tdTomato-cNLS

    Plasmid

    #138532

    Purpose

    Lentiviral inducible expression of tdTomato with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TfR-mCh-DHFR

    Plasmid

    #173013

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-mCh-DHFR
    

    Tags

    DHFR and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. RHBDD1 pmRFP-N2 (1390)

    Plasmid

    #62049

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. PB_EF1a-CasRx-msfGFP-2A-Blast

    Plasmid

    #226010

    Purpose

    CasRx protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    CasRx RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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