169,450 results
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Plasmid#225876PurposeGuides targeting TP53 and RB1DepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only
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hzHDAC10
Plasmid#225542PurposeExpresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.DepositorInsertHistone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationMutated A24E and D94APromoterT7Available SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro HA-hUSP27X Y381H
Plasmid#225723PurposeTransfection of USP27X (Y381H) with HA tag in mammalian cellsDepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH504
Plasmid#215782PurposeNBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC geneDepositorInsertsLacI(YQR)
sgRNA-ribozyme cassette
dCas9
UseSynthetic BiologyExpressionBacterialPromoterBt1311, P(O1), and P1Available SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_ARPC1B:1-372
Plasmid#211195PurposeMammalian expression of full-length human ARPC1B. N-terminal HiBiT tag.DepositorInsertARPC1B:M1-K372 (ARPC1B Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CSII-CAG-EGFP
Plasmid#214112PurposeMammalian continuous expressionDepositorInsertEGFP
UseLentiviralExpressionMammalianMutationCodon-optimized for mammalian cellsAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_ARPC1B:1-372
Plasmid#211268PurposeMammalian expression of full-length human ARPC1B. C-terminal HiBiT tag.DepositorInsertARPC1B:M1-K372 (ARPC1B Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF cyto-cmCh
Plasmid#188903PurposeExpresses cytosolic mCherryDepositorInsertcytosolic mCherry
ExpressionMammalianAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin-TEV-His6
Plasmid#178067PurposeInsect cell expression of gamma-tubulin-TEV-His6DepositorAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab6a
Plasmid#109652PurposeMuscle specific zebrafish expression of mKate2 fused to rab6aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab6ba
Plasmid#109653PurposeMuscle specific zebrafish expression of mKate2 fused to rab6baDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab6bb
Plasmid#109654PurposeMuscle specific zebrafish expression of mKate2 fused to rab6bbDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbA19k-RFP
Plasmid#126105PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23105Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH3
Plasmid#83553PurposeUsed to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.DepositorInsertHXT7 promoter-yEGFP
ExpressionYeastPromoterHXT7Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro
Plasmid#31482DepositorInsertTraffic Light Reporter 1.1 (Sce Target) EF Puro
UseLentiviralMutationmCherry has M9S and M16L mutations. This is what …Available SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1
Plasmid#125840Purposeexpress wild type BAP1DepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
BirA pLEMO
Plasmid#119818PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
ExpressionBacterialPromoterRhamnose promoterAvailable SinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.19_PB-CIT-PGK-Blast
Plasmid#205564PurposeExpress human CITDepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2/9 REP-AAP-ΔCAP
Plasmid#205991PurposeRep and AAP protein expression for AAV CREATE librariesDepositorInsertAAV9 Rep and AAP proteins for CREATE libraries
UseAAVAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
GreenGate
Plasmid Kit#1000000036PurposeGolden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassetteDepositorAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
PX458-mcherry
Plasmid#161974PurposeExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNADepositorInsertCas9-t2a-mcherry
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG2(11)1-10
Plasmid#82610PurposeLentiviral vector of constitutive expression of mNeonGreen2(1-10)DepositorInsertmNeonGreen2(1-10)
UseLentiviralExpressionMammalianMutationK128M, S142T, R150M, G172V and K213MPromoterSFFVAvailable SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (AAV Retrograde)
Viral Prep#84445-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (#84445). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] plasmid DNA. CAG-drive, Cre-dependent Jaws-GFP for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFP (Cre-dependent)Available SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
TagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer X-null replicon
Plasmid#65461Purposecontaining 1.3 units of the X-null HBV genome (subtype ayw)DepositorInsert1.3 units of the X-null HBV genome
ExpressionMammalianAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-pSu9-HaloTag7-mGFP
Plasmid#184905PurposeStable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activityDepositorInsertPresequence of Fo-ATPase subunit 9
TagsHaloTag7 and monomeric EGFPExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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EGFP-Tau(WT)
Plasmid#187023PurposeExpresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoterDepositorAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-SIRT1
Plasmid#1791PurposeMammalian expression of flag-tagged SIRT1DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)
Plasmid#217306PurposesgRNA mcherry + PuromycinDepositorInsertpU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
UseCRISPRPromoterU6, Ef1alphaAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only