165,839 results

1. pEGFPc1 IP3R3 WT

   Plasmid

   #236453

   Purpose

   transient overexpression of IP3R3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

   Insert

   IP3R3 (Itpr3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3puro-mGFP-ROCK2

   Plasmid

   #225472

   Purpose

   Express mEGFP-fusion protein of ROCK2

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMT3_SRSF1

   Plasmid

   #201056

   Purpose

   Express sumo-tagged human SRSF1

   Depositor

   Jun Zhang

   Article

   Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

   Insert

   SRSF1 (SRSF1 Human)
   

   Use

   Tags

   sumo

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCX419

   Plasmid

   #229216

   Purpose

   All-in-one plasmid for labeling of XIST RNA via Csm complex

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV4 p50

   Plasmid

   #21965

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NF-kB p50 subunit (NFKB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 MDM2 WT

   Plasmid

   #16233

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6a:sgRNA#1

   Plasmid

   #64245

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. peGFP-N1-Cofilin

   Plasmid

   #186747

   Purpose

   Expresses wildtype GFP-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Lamin-A

   Plasmid

   #206027

   Purpose

   Expresses GFP-tagged Lamin A in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Lamin-A (LMNA Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD5

    Plasmid

    #73914

    Purpose

    multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    dU6-3:tRNA:gRNA:tRNA:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a_6H-MMLV_RT_D524N-6H

    Plasmid

    #166945

    Purpose

    Bacterial expression of M-MLV reverse transcriptase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    gag-pol
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D524N, H8Y

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN(CMV-copGFP-Puro-SMAR)

    Plasmid

    #80391

    Purpose

    expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Puromycine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVE-eGFP

    Plasmid

    #52581

    Purpose

    Lentiviral transfer vector driving expression of eGFP in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-HA-NLS-I-PpoI

    Plasmid

    #46963

    Purpose

    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    NLS-I-PpoI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TET

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. LifeAct-mNeonGreen

    Plasmid

    #98877

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    LifeAct
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIE1

    Plasmid

    #136355

    Purpose

    Selection by Inducible Effectors

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    P1TDP-GHO23

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-FLAG-SF2

    Plasmid

    #99021

    Purpose

    Mammalian expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2-EF1a-GFP1-10-IRES-Puro

    Plasmid

    #211467

    Purpose

    Lentivirus backbone expressing GFP1-10

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA3-HA-human MYCN

    Plasmid

    #74163

    Purpose

    Mammalian expression of human MycN

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pPSU150

    Plasmid

    #173836

    Purpose

    Expresses 150 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEMS-II

    Plasmid

    #204765

    Purpose

    GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    EGFP-m6Alinker-DHFR
    

    Use

    Tags

    Expression

    Mutation

    mutated all RACs in EGFP to RGC/RCC

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

    Plasmid

    #100848

    Purpose

    AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1 and AAV9

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

    Plasmid

    #232880

    Purpose

    Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    ADP-ribosylation factor-like protein 13B (ARL13B Human)
    

    Use

    Lentiviral

    Tags

    GFP and RGECO1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-Cre

    Plasmid

    #198712

    Purpose

    For lentiviral expression of Cre recombinase in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Insert

    Pgk-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTMB352_ZFcharm_Kv1_split-inteins

    Plasmid

    #220848

    Purpose

    Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv2
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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