We narrowed to 6,990 results for: Cad

1. pRK5-HA-Parkin

   Plasmid

   #17613

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209665

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74009

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5x UAS
   Cas9
   T2A
   GFP
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL2- Empty control -SV40-Luc

   Plasmid

   #26280

   Purpose

   Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   opposing T7 promoters

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #236406

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFCIV-CEACAM6

   Plasmid

   #149303

   Purpose

   lentiviral packaging vector for CEACAM6 expression.

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ubiquitin C

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. piRFP-N3-Tyrosinase

   Plasmid

   #80152

   Purpose

   Melanosome marker

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

   Insert

   Tyrosinase (TYR Human)
   

   Use

   Tags

   iRFP682

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLV-CAG-FLEX-BFP2-(mCherry)’

   Plasmid

   #115234

   Purpose

   Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   mTagBFP2 and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGE32

    Plasmid

    #63159

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. HaloTag-ER-3

    Plasmid

    #186960

    Purpose

    ER lumen localizaing HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CALR (CALR Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209653

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95A.A119L

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET24a-VHH-std

    Plasmid

    #109417

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5 mSynCAM3

    Plasmid

    #115498

    Purpose

    expression of mouse full-length SynCAM 3

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm3 (Cadm3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLIK-Venus

    Plasmid

    #25734

    Purpose

    Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLNCX2-mEGFP-TSG101

    Plasmid

    #116925

    Purpose

    expresses mEGFP-TSG101 in mammalian cells

    Depositor

    Sanford Simon

    Article

    Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

    Insert

    TSG101 (TSG101 Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Klotho (secreted)

    Plasmid

    #17713

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPR_zeo_sgNT135

    Plasmid

    #183181

    Purpose

    pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET32a-TRXtag

    Plasmid

    #11516

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    None - thioredoxin is vector encoded (trxA )
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    vector modified; removed N-terminal His tag by di…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pBAD-mGreenLantern

    Plasmid

    #161913

    Purpose

    mGreenLantern fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209652

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FEMP

    Plasmid

    #191973

    Purpose

    FRET-based indicator of ER-mitochondria proximity (FEMP)

    Depositor

    Luca Scorrano

    Article

    Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    AkapYFP-Tav2a-CFP-sac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-Cyclin D1

    Plasmid

    #19927

    Depositor

    Yue Xiong

    Article

    Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

    Insert

    Cyclin D1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FRB-NLuc

    Plasmid

    #31181

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

    Plasmid

    #40640

    Depositor

    Brenda Lilly

    Article

    Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

    Insert

    NICD3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLIK-Hygro

    Plasmid

    #25737

    Purpose

    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDF-1b-RFC140ΔN555

    Plasmid

    #175043

    Purpose

    Overexpression of human (Rfc1) p140ΔN555 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunit 1 (RFC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209659

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S29W.A95K

    Promoter

    GFAP

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge