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Showing: 501 - 550 of 1071 results

  10. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    atf4 (ATF4 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226105-plasmid-map-sequence-id-448414

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  11. pCS2-Opto-Acvr1ba-P2A-Acvr2ba

    Plasmid
    #232640
    Purpose
    Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression
    Depositor
    Katherine Rogers
    Insert
    Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    232640-plasmid-map-sequence-id-461338

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  27. GSK-3beta S9A (Xenopus)/pCS2+

    Plasmid
    #16333
    Depositor
    Peter Klein
    Insert
    GSK-3beta (gsk3b.L Frog)
    Use
    Vertebrate expression
    Tags
    Expression
    Mutation
    GSK-3 with serine-9 to alanine mutation
    Promoter
    Available since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only

  28. GSK-3beta S9A (Human)/pCS2+

    Plasmid
    #16334
    Depositor
    Peter Klein
    Insert
    glycogen synthase kinase-3 beta (GSK3B Human)
    Use
    Vertebrate expression
    Tags
    Myc
    Expression
    Mutation
    GSK-3 beta with serine-9 to alanine point mutation
    Promoter
    Available since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only

  32. XE173 mouse Wnt-1-myc-pCS2+

    Plasmid
    #16851
    Depositor
    Randall Moon
    Insert
    Wnt-1 (Wnt1 Mouse)
    Use
    Xenopus expression
    Tags
    myc
    Expression
    Mutation
    Promoter
    Available since
    March 14, 2008
    Availability
    Academic Institutions and Nonprofits only

  35. GSK-3beta-his (Xenopus)/pCS2+

    Plasmid
    #16335
    Depositor
    Peter Klein
    Insert
    glycogen synthase kinase-3beta (gsk3b.L Frog)
    Use
    Vertebrate expression
    Tags
    6xHis
    Expression
    Mutation
    Xenopus GSK-3 with 6X his tag
    Promoter
    Available since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only

  39. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid
    #230996
    Purpose
    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.
    Depositor
    Michael Rape
    Article
    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:
    Type
    Empty backbone
    Use
    Tags
    AcGFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    230996-plasmid-map-sequence-id-457945

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  40. pcs2-cDELE1(142-515)-sumo

    Plasmid
    #226107
    Purpose
    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    DELE1 (DELE1 Human)
    Use
    Tags
    Sumo
    Expression
    Mammalian
    Mutation
    encodes only residues 142-515 of DELE1 (represent…
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226107-plasmid-map-sequence-id-449375

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  46. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid
    #154910
    Purpose
    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.
    Depositor
    Jonathan Clarke, Clare Buckley
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    Phytochrome B (PHYB Mustard Weed, Synthetic)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB
    Promoter
    CMV
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154910-plasmid-map-sequence-id-318440

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Showing: 501 - 550 of 1071 results