We narrowed to 13,119 results for: 112
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Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)
ExpressionYeastPromoter5 XREs+ minimal Cytochrome CAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Raptor Halo
Plasmid#112754Purposeexpress Raptor-HaloDepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
Available SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LSS-SGFP2
Plasmid#112943PurposeProduces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRETDepositorInsertLSS-GFP2 mCherry fusion
ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCa-C1
Plasmid#112264PurposeITRACKalpha Donor ConstructDepositorInsertprotein kinase C alpha (PRKCA Bovine)
TagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE30-mBFP
Plasmid#112682PurposeExpression of NADPH-dependent blue fluorescent proteinDepositorInsertHis-mBFP
TagsHis tagExpressionBacterialPromoterT5Available SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABEmax_3xHA
Plasmid#112098PurposeA:T-to-G:C base editingDepositorInsertABEmax_3xHA
UseCRISPRTags3xHAExpressionMammalianPromoterCMVAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LUC01 (L/Sec/P)
Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
ExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:Ble:Cas9
Plasmid#112208PurposeGolden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.DepositorInsertsCas9
SheBle
UseCRISPR; Diatom, phaeodactylumTagsPhaeodactylum fcp promoter and terminator and YFPMutationPhaeodactylum Fcp promoterPromoterPhaeodactylum FcpAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMal_Abl-PRM 3R
Plasmid#112086PurposeBacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.DepositorInsertAbl-PRM-3R (ABL1 Human)
TagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
RhoA sensor (LSS-SGFP2-mCherry)
Plasmid#112948PurposeRhoA FRET biosensorDepositorInsertRhoa sensor
TagsLSS-SGFP2 and mCherryExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS428
Plasmid#11257DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-delC
Plasmid#112493PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
TagsFlagExpressionMammalianMutationdeletion of C terminus (AA290-671 deleted)Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag
Plasmid#112634PurposeTwo SpyTags linked by an elastin-like polypeptide for hydrogel formationDepositorInsertSpyTag-ELP-SpyTag
TagsHis6ExpressionBacterialMutationcentral cysteinePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2
Plasmid#112004PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mKate2DepositorHas ServiceAAV9Insertaxon-GCaMP6s-P2A-mKate2
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianPromoterhSynapsin1Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH3(2)-3R
Plasmid#112091PurposeBacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.DepositorAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-2(KW)2-eGFP
Plasmid#112736PurposeExpress 2(KW)2-eGFP in E. coliDepositorInsert2(KW)2-eGFP
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1661
Plasmid#112035PurposeOsTIR1 expression cassette marked with URADepositorInsertTIR1
ExpressionYeastAvailable SinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only