We narrowed to 1,042 results for: Psp
-
Plasmid#79054PurposeExpresses SpyTagged GlpG proteinDepositorInsertSpyTag-GlpG (glpG )
Tags6xHis and SpyTagExpressionBacterialMutationGlpG protein employed here corresponds to residue…PromoterTrcAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t-gfp
Plasmid#48130PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXB3 (human) FLAG pSP65
Plasmid#8523DepositorInsertHOXB3 (HOXB3 Human)
UseIn vitro transcription/translationTagsFLAGExpressionBacterialMutationContains 5 additional amino acids before ATG from…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccC
Plasmid#158112Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
ExpressionMammalianAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccA
Plasmid#158114Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
ExpressionMammalianAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP019
Plasmid#168195Purposeexpresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.DepositorInserteatA[H134R]
ExpressionBacterialMutationH134R mutationPromoteraraBADAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_U6_sgRNA_Mettl3C
Plasmid#165420PurposesgRNA construct for targeting Mettl3 C-terminusDepositorAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpec
Plasmid#60593Purposeused with pAND_YFP to generate an AND gate without memoryDepositorInsertSpec
ExpressionBacterialAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPVpr-hp
Plasmid#48119PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoterDepositorInsertsignal peptide of Vpr
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPSP6
Plasmid#48150PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
TagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cmv d0 dPspCas13b-GGS-Sun
Plasmid#119858PurposeCmv d0 dPspCas13b-GGS-Sun no-fusion constructDepositorInsertdCas13b-GGS-Sun
UseCRISPRExpressionMammalianAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSport6/FLAG-Wwtr1(Taz)
Plasmid#27318DepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSpot-Tag-Lamin
Plasmid#117062PurposeFor mammalian expression of lamin B1 with N-terminal Spot-TagDepositorAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-GFP (PX458)
Plasmid#129025Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP
Plasmid#198863PurposePlasmid encoding for 2 gRNAs targeting the human TERT geneDepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting
Plasmid#188541PurposePlasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codonDepositorInsertgRNA
UseCRISPRTagsGFPPromoterU6Available SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP64T acvr1ba*
Plasmid#185409PurposeConstitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)DepositorInsertacvr1ba (acvr1ba Zebrafish)
UseIn vitro synthesis of mrnaExpressionMammalianMutationchanged Threonine 206 to Aspartic acidAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-CPspaH2B
Plasmid#133385PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBP-Pspac
Plasmid#112778PurposePromoter repressed by LacI (not natively produced in B. subtilis 168)DepositorArticleInsertPspac promoter
ExpressionBacterialAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only