We narrowed to 11,250 results for: streptococcus -(crispr) -(cas9)

Showing: 2181 - 2200 of 11250 results

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dead Sa Cas9

Plasmid

#138162

Purpose

For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

dSaCas9

Tags

NLS

Expression

Mammalian

Mutation

D10A + N580A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-eBE-Y130F

Plasmid

#113423

Purpose

Expresses hA3A-eBE-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCP-GFP

Plasmid

#101161

Purpose

to epress PCP-GFP fusion protein

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

PCP-GFP

Use

Lentiviral

Promoter

Ubc

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCBC-MT2T3

Plasmid

#50594

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T2, gRNA scaffold, TaU3t plus, U6-26p, T3

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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AYBEv3

Plasmid

#193967

Purpose

An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

ecTadA8e-nSpCas9(D10A)-MPG(v3)

Expression

Mammalian

Mutation

G163R, N169S, S198A, K202A, G203A, S206A, K210A

Promoter

hU6, EF1a, CBh

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ154

Plasmid

#69305

Purpose

Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9 (Arabidopsis codon optimized)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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M3-M14-dCas9

Plasmid

#134779

Purpose

Expression of fusion protein M3-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 Human, S. pyogenes)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ146

Plasmid

#69298

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABEmax

Plasmid

#119815

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.2

Plasmid

#190796

Purpose

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.0

Plasmid

#190795

Purpose

PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

PRE-Hsp70Bb-Cas9-T2A-eGFP

Use

CRISPR

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSimpleII-NmCas9-FLAG

Plasmid

#47872

Purpose

FLAG tagged NmCas9 with no NLS.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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U6.3>Control.gRNA.f+e

Plasmid

#99140

Purpose

Control gRNA under the regulation of an optimized chicken specific U6 promoter

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

Control gRNA

Use

CRISPR

Promoter

Chicken U6.3

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCBC-MT3T4

Plasmid

#50595

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T3, gRNA scaffold, U6-26t plus, OsU3p, T4

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-ABE(7.10)

Plasmid

#108387

Purpose

Mammalian expression vector for xCas9(3.6)-ABE

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-ABE(7.10)

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA-ura-HYB

Plasmid

#64330

Purpose

ura3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of ura3 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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