We narrowed to 10,414 results for: 158

1. pcDNA3-ALK5 K232R

   Plasmid

   #80878

   Purpose

   mammalian expression of ALK5 K232R

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K232R (kinase dead)

   Promoter

   CMV

   Available since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-P4:gs:nls:VPR

   Plasmid

   #135983

   Purpose

   Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the VPR activation domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-UAS-mIFNγ

   Plasmid

   #230004

   Purpose

   Gal4-dependent expression of secreted mouse IFNγ

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   UAS-mIFNγ (Ifng Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UAS

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAF0443 Nm60_attP-mCherry donor

   Plasmid

   #193471

   Purpose

   Promoterless mcherry donor for Nm60

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCEP4-MR1

   Plasmid

   #178603

   Purpose

   Mammalian expression of myc-tagged MR1

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TDP43 NOTAG2

   Plasmid

   #28207

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa86-414 only

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Myc:P4:gs:nls:ABI

   Plasmid

   #135985

   Purpose

   Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the ABI domain
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SaCas9 TadDE

   Plasmid

   #193846

   Purpose

   Express TadDE (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE with SaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAF0466 Ef1a-Nm60_attB acceptor

   Plasmid

   #193476

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60_attB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-h-mmPtch1-FL

    Plasmid

    #120889

    Purpose

    Expresses wildtype Ptch1 for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3/Myc-DNMT3B3

    Plasmid

    #37546

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B3 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRE3G-iRFP-B1-timestamp_A

    Plasmid

    #159924

    Purpose

    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-GfaABC1D-GRAB_eCB2.0

    Plasmid

    #164609

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    GfaABC1D

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pv6_EGFP

    Plasmid

    #179392

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    EGFP
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3/Myc-DNMT3B2

    Plasmid

    #36942

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B2 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pWay21-MKP-1 FL

    Plasmid

    #13469

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1 FL (Dusp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pMIR-His-Halo-Tev-PTEN

    Plasmid

    #58241

    Purpose

    expresses halo tagged PTEN protein

    Depositor

    Yimon Aye

    Article

    Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

    Insert

    phosphatase and tensin homolog (PTEN Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-Merlin S518A

    Plasmid

    #84294

    Purpose

    Expression of Merlin with an alanine mutation at the Pak phosphorylation site

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S518A

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cry2L348F(1-531)-mCh-BAXS184E

    Plasmid

    #117239

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531.L348F-mCh-BAXS184E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CREB3L3

    Plasmid

    #99509

    Purpose

    to express CREB3L3 in mammalian system

    Depositor

    Dong-Yan Jin

    Article

    Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

    Insert

    CREB3L3 (Creb3l3 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRN110

    Plasmid

    #127228

    Purpose

    CmYLCV::STM::35S terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    STM (STM Mustard Weed, A. tumefaciens)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-Merlin S518D

    Plasmid

    #84295

    Purpose

    Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S518D

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJFRC-20XUAS-IVS-PhiC31

    Plasmid

    #133565

    Purpose

    Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

    Plasmid

    #191145

    Purpose

    Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3UTR(Bdnf)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. eNme2-C TadDE

    Plasmid

    #193842

    Purpose

    Express TadDE (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadDE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-SI-121

    Plasmid

    #131126

    Purpose

    A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

    Plasmid

    #191141

    Purpose

    Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-W:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    UAG>UGG (stop>Trp)

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

    Plasmid

    #191159

    Purpose

    Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:EGFP.s2:mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJSK418

    Plasmid

    #205131

    Purpose

    Expression of GFP-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA HisB TO rFez1

    Plasmid

    #17385

    Depositor

    Stephen Goff

    Article

    Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

    Insert

    FEZ1 (Fez1 Rat)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only