We narrowed to 35,025 results for: Spr;
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Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRTagsExpressionBacterial and InsectMutationPromoter3XP3 eye-expression PromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
piCRg Entry
Plasmid#58904PurposeCas9/gRNA Entry expression plasmidDepositorInserthSpCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.csr-1.Y-SUP4t
Plasmid#68060PurposegRNA for csr-1 locus in N. crassaDepositorInsertgRNA for csr-1 locus
UseCRISPR; N. crassa, fungiTagsExpressionYeastMutationPromoterSNR52Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PU6::klp-12_sgRNA
Plasmid#46170Purposeklp-12 targeting sgRNADepositorInsertklp-12 targeting sgRNA (klp-12 Synthetic)
UseCRISPRTagsExpressionWormMutationPromoterC. elegans U6 snRNA pol III promoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA P2
Plasmid#80944PurposeExpresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 bindingDepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) TFRC
Plasmid#74709PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNADepositorInsertU6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A2.0
Plasmid#99896PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNW3
Plasmid#53372PurposeCsy4 and Cas9 D10A nickase expression plasmidDepositorInsertCsy4-T2A-Cas9n (D10A)
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pADH147
Plasmid#90991PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.DepositorInsertgRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH119
Plasmid#90985PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG80
Plasmid#89282PurposeEntry vector containing TaCas9-nos terminator between attL1 and attR5 sitesDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_TEAD1_1
Plasmid#86356PurposeEncodes gRNA for 3' target of human TEAD1DepositorInsertgRNA against TEAD1 (TEAD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M1S_AmpR
Plasmid#119153PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M1NS_AmpR
Plasmid#119155PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCasD10A-pA
Plasmid#51638PurposeExpresses Cas9-nickase in mammalian cells and zygotes.DepositorInsertCodon-optimized Cas9 D10A
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianMutationPromoterCAG promoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_Tet-inducible Luciferase reporter
Plasmid#64161PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.DepositorInsertsgRNA1 for Tet-inducible Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only