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Plasmid#226202PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).DepositorInsertHomology arms for soluble hydrogenase operon
UseSynthetic BiologyTagsExpressionMutation∆hoxFUYHWI ∆hypA2B2F2PromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC023 (∆MBH)
Plasmid#226201PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).DepositorInsertHomology arms for membrane bound hydrogenase operon
UseSynthetic BiologyTagsExpressionMutation∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC022 (∆phcA)
Plasmid#226200PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC004 (∆phaCAB)
Plasmid#226198PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 15, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC027 (∆pemK)
Plasmid#226204PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.DepositorInsertUpstream/downstream homology arms for addiction system toxinPemK.
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pT3TS-spCas9 ABE-Umax-ex1
Plasmid#222141PurposeAdenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2+spCas9 ABE-Umax-nanos
Plasmid#222142PurposeAdenine base editor with modified TadA variant for germline A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9 with nanos UTR
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterSP6Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NPM2-T2A-mGreenLantern-PuroTK
Plasmid#222910PurposeHomology directed repair template for knocking in mGreenLantern reporter to NPM2.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR_FIGLA-T2A-mGreenLantern-PuroTK
Plasmid#222905PurposeHomology directed repair template for knocking in mGreenLantern reporter to FIGLA.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR2_TFAP2C-T2A-mGreenLantern-Hygro
Plasmid#222915PurposeHomology directed repair template for knocking in mGreenLantern reporter to TFAP2C.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-PRDM1
Plasmid#222924PurposePiggyBac transposon plasmid for doxycycline inducible expression of PRDM1DepositorInsertPRDM1 (PRDM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-ZGLP1
Plasmid#222926PurposePiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1DepositorInsertZGLP1 (ZGLP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERH-SOHLH1
Plasmid#222922PurposePiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1DepositorInsertSOHLH1 (SOHLH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax-ex2-rest1
Plasmid#222143PurposeAdenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax-ex1-rest1
Plasmid#222140PurposeAdenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
opt.a_pSTC0-zeo
Plasmid#202347PurposeDesign opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertopt.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
opt.b_pSTC0-zeo
Plasmid#202348PurposeDesign opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertopt.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
neg.ctrl_pSTC0-zeo
Plasmid#202350PurposeControl neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertneg. ctrl
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Q54A
Plasmid#217912PurposeA plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutationDepositorInsertPXP Q54A
UseTags6x His (N and C term)ExpressionBacterialMutationGlutamine at position 55 and 267 mutated to Alani…PromoterT5Available sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
T40A
Plasmid#217911PurposeA plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutationDepositorInsertPXP T40A
UseTags6x His (N and C term)ExpressionBacterialMutationThreonine at position 41 and 253 mutated to Alani…PromoterT5Available sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-DIO-GCaMP6f-WPRE
Plasmid#216275PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6fDepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-GCaMP6f-WPRE
Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)
Plasmid#208869PurposeFor mammalian expression of NAP1 (delta-TBK1)DepositorInsertNAP1 (AZI2 Human)
UseTagsHAExpressionMammalianMutationL226Q/L233Q (disrupts TBK1 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-GFP-NAP1
Plasmid#208870PurposeFor expression in E. coli of GFP-NAP1DepositorInsertNAP1 (AZI2 Human)
UseTagsGFP and GST-TEVExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL6-H2B-iRFP720-bla
Plasmid#189991PurposeLentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistanceDepositorInsertHuman histone H2B
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45
Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorInsertCdc45 (cdc45.L Frog)
UseTagsFLAG and HisExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-WM
Plasmid#208798PurposeXenopus laevis His6-Myc-DONSON (W234L, M463T)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationW234L; M463TPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-S437A
Plasmid#208803PurposeXenopus laevis His6-Myc-DONSON (S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationS437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-ΔD1
Plasmid#208804PurposeXenopus laevis His6-DONSON (1-155aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHisExpressionBacterialMutationΔD1, deletion of 1-155aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-GFP-T2A
Plasmid#203608PurposeDonor plasmid to create 5' knockin of NKX2-5 with GFP-T2ADepositorInsertNKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
UseHuman targetingTagsExpressionMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.HALO-KIF3A tail 419-702
Plasmid#193924PurposeMammalian expression of Halo-tagged Kif 3A tail 419-702DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only