We narrowed to 10,362 results for: Coli
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Plasmid#216749PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [FKISLAEMā¦Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL2del
Plasmid#216750PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [WMGILKNNā¦Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL3del
Plasmid#216751PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 3 (OL3) with the sequence of [ELLGGPDSā¦Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL3/2sub
Plasmid#216752PurposeStudy the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [WMGILKNNā¦Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinD-IRES-Puro
Plasmid#213114PurposeExpression of untagged MinD in mammalian cellsDepositorAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM253 (miniCTX1-rhaSR-PrhaBAD)
Plasmid#110571PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.657
Plasmid#108525PurposeCymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.DepositorInsertCymRAM + PCymRC-YFP
ExpressionBacterialMutationCymR: S110G A171VAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Flp
Plasmid#122969PurposeExpresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.DepositorInsertFlp recombinase
ExpressionBacterialPromoterpBADAvailable SinceAug. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-Tn5
Plasmid#60240PurposeIPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.DepositorInserttnpA from pBam1
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7 promoterAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRT802
Plasmid#68113PurposeIntegration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
aph11
oriT
attP
int
UseStreptomyces integrating vectorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
ExpressionBacterialMutationAraC: L133F E165L E169G C280LAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)-c-term-cpGFP-ERexp
Plasmid#195346PurposeControl plasmid for the MscL tension biosensorDepositorInsertMscL(G22S)-c-term-cpGFP-ERexp
UseLentiviralExpressionMammalianPromoterCMVd2Available SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-RF2
Plasmid#124130PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
secreted BirA-Flag
Plasmid#64395PurposeFlag-tagged biotinylation enzyme for co-expression with bio-proteinsDepositorAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorMutationFragmentAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJM.661
Plasmid#108532PurposeTtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.DepositorInsertTtgRAM + PTtg-YFP
ExpressionBacterialMutationTtgR: R130H A144T A148VAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-bleo-atpD-K155Q
Plasmid#248068PurposeLentiviral expression of a catalytically inactive mutant of the beta subunit of ATPGobble. Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for a LOF control.DepositorInsertatpD
UseLentiviralTagsFLAGMutationLysine 155 to Glutamine, codon-optimized for exprā¦PromoterTRE3GSAvailable SinceNov. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-bleo-atpD
Plasmid#248066PurposeLentiviral expression of beta subunit of ATPGobble. Includes ampR and bleoR (zeocin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for active ATPGobble.DepositorInsertatpD
UseLentiviralTagsFLAGMutationcodon-optimized for expression in human cellsPromoterTRE3GSAvailable SinceNov. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-bsd-atpA
Plasmid#248065PurposeLentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.DepositorInsertatpA
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsPromoterTRE3GSAvailable SinceNov. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
SepOTSĪ»
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
TagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXint129
Plasmid#178208PurposeEncodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.DepositorExpressionBacterialPromoterlacIAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLS.601
Plasmid#184955PurposeExpress -Eco1 ncRNA library variantsDepositorInsertEco1 ncRNA (variants)
ExpressionBacterialMutationLibrary members with mutations described in manusā¦PromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF463
Plasmid#167671PurposeIntermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2ST
Plasmid#59384PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.DepositorInsertTT-ISceI-dfrA
UseTemplateAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MTF-His
Plasmid#124133PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG006 (Ec67 retron)
Plasmid#157884PurposeExpresses the Ec67 retron defense systemDepositorInsertEc67 retron
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRecE564T
Plasmid#226825PurposeExpressing RecE*T controled by Ptrc promoterDepositorInsertrecE*T
ExpressionBacterialAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only