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pMBD-Gate2

Plasmid

#25947

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

GAL4 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pHIE041 CAG mKate2 (TUPV 1)

Plasmid

#138721

Purpose

mMoClo TUPV, with CAG promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

CAG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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TRPML2-MYC

Plasmid

#18829

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pscPP7(2xWT)-EGFP

Plasmid

#140261

Purpose

PP7CP-dependent translational repression

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Two PP7CP-binding sites embedded in scaffold

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_TagBFP

Plasmid

#62400

Purpose

MXS_chaining vector with TagBFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

TagBFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-RA-HRas-IRES2-Lyn-iRFP682

Plasmid

#180584

Purpose

RA-Hras and Lyn-iRFP682

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

RA-Hras and Lyn-iRFP682

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-15

Plasmid

#119994

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0285

Plasmid

#11304

Depositor

Sung-Hou Kim

Article

Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

Insert

small heat shock protein (MJ_0285 M. jannaschii)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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MXS_iRFP670

Plasmid

#62411

Purpose

MXS_chaining vector with iRFP670

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

iRFP670

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pMOX

Plasmid

#160922

Purpose

Methanol oxidase (MOX) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Methanol oxidase promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2clust-eGFP-2xC2-iLID

Plasmid

#114420

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_mAzamiGreen

Plasmid

#62404

Purpose

MXS_chaining vector with mAzamiGreen

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mAzamiGreen

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-22

Plasmid

#120001

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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WT Nedd4L

Plasmid

#200086

Purpose

Nedd4L in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

Nedd4L (NEDD4L Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-11

Plasmid

#119990

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HF1

Plasmid

#104173

Purpose

Cas9-HF1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-HF1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2B -158/+64

Plasmid

#24946

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-NAD

Plasmid

#58345

Purpose

pEGFP-Mirt with a short intron from NDUFS1 cloned in

Depositor

Matthew Wood

Article

Seow et al RNA. 2012 Jul;18(7):1328-37. doi: 10.126

Insert

NDUSF1 intron 6 (NDUFS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pPD554 ZF129x6-Compact_EYFP

Plasmid

#138868

Purpose

EYFP reporter vector for the ZF129x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF129x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF129x6-Compact

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPD535 VP16-ZF129

Plasmid

#138768

Purpose

Constitutive expression of VP16-ZF129 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF129

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) Full length Her2(ERBB2)-Fc

Plasmid

#194330

Purpose

Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

Depositor

Alexander McLellan

Insert

Her2-Fc (ERBB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_mCherry

Plasmid

#62408

Purpose

MXS_chaining vector with mCherry

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mCherry

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_mKO2

Plasmid

#62406

Purpose

MXS_chaining vector with mKO2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mKO2

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD303 VP64-ZF43

Plasmid

#138835

Purpose

Constitutive expression of VP64-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBORF-DBD

Plasmid

#127463

Purpose

ORF filtering plasmid (lambda cI associated plasmid for pAVA)

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPD158 dsRedExpress2-ZFi37

Plasmid

#138797

Purpose

Constitutive expression of dsRedExpress2-ZFi37 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

dsRedExpress2-ZFi37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_rtTA3

Plasmid

#62428

Purpose

MXS_chaining vector with rtTA3

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Reverse tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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MTK3a_007

Plasmid

#123760

Purpose

Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-RNF17L

Plasmid

#35413

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17l (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
2xsspB-mSc-sspB-Tiam1

Plasmid

#114424

Purpose

Translocating Tiam1 to iLID and activate Rac1

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

Tiam1

Tags

mScarlet

Expression

Mammalian

Mutation

truncated Tiam1 (64-437)

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

Plasmid

#87584

Purpose

PV5 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
epiCRISPRn

Plasmid

#135961

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_LSS-mKate2

Plasmid

#62402

Purpose

MXS_chaining vector with LSS-mKate2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

LSS-mKate2

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_mEGFP

Plasmid

#62403

Purpose

MXS_chaining vector with mEGFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mEGFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM584 ZF2x6-C mKate2 in TUPV1

Plasmid

#161528

Purpose

Inducible expression of mKate2 under the ZF2x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF2x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mVermilion-C1

Plasmid

#105756

Purpose

Empty vector for creating N terminal mVermilion fusions

Depositor

Megan Rizzo

Article

Lee et al Elife. 2021 Oct 29;10. pii: 67858. doi:

Type

Empty backbone

Tags

mVermilion

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

Plasmid

#114726

Purpose

dCas9-VP160 expression in mammalian cells

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

dCas9-VP160

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-06

Plasmid

#119985

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with sfGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

sfGFP

Use

Pcr template

Tags

sfGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 AU1 mTOR S2035W

Plasmid

#15678

Depositor

John Lawrence

Article

McMahon et al Mol Cell Biol. 2002 Nov . 22(21):7428-38

Insert

mTOR (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

S2035W

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pIM572

Plasmid

#74640

Purpose

alias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-iGECInano

Plasmid

#186189

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Bacterial

Promoter

AraC

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_Kaede

Plasmid

#62413

Purpose

MXS_chaining vector with Kaede

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Kaede

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM9121_pFMD

Plasmid

#160921

Purpose

Formate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Formate dehydrogenase promoter

Use

Synthetic Biology

Mutation

BsaI and BbsI domesticated

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Puro Empty (w609-1)

Plasmid

#20957

Purpose

3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Puro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
iLID-eGFP-2xC2-iLID

Plasmid

#114418

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTGMP-p16/p19.478

Plasmid

#32717

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

p16/p19

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
pBORF-AD

Plasmid

#127462

Purpose

ORF filtering plasmid (RNAp associated plasmid for pAVA)

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEFIRES NQO1 Y128F

Plasmid

#61734

Purpose

Expresses human Y128F NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Mutation

Y128F

Promoter

Ef1a

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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