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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,945 results for: lif

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Showing: 4921 - 4960 of 7945 results

  7. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  14. pXWJ109As(RS-te-AAV)

    Plasmid
    #123156
    Purpose
    For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, ParsR, PhrpLE
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123156-plasmid-map-sequence-id-240600

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  17. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85931-plasmid-map-sequence-id-172473

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  20. pPI304

    Plasmid
    #113232
    Purpose
    extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP
    Depositor
    Rob Kay
    Article
    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do
    Insert
    LifeAct & pkgE-PH
    Use
    Dictyostelium discoideum expression vector, extra…
    Tags
    mCherry & GFP
    Promoter
    act15
    Available Since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113232-plasmid-map-sequence-id-217859

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  22. pOPO717

    Plasmid
    #195302
    Purpose
    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and lacZ spacer 5 in native array
    Expression
    Bacterial
    Promoter
    Arabinose inducible arapBAD
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195302-plasmid-map-sequence-id-383138

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  23. roGFP_iE_C147S_pQE30

    Plasmid
    #48631
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, C147S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48631-plasmid-map-sequence-id-70379

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  31. As2

    Plasmid
    #123160
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, ParsR-ABS84, PhrpLE
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123160-plasmid-map-sequence-id-243993

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  34. EGFP–COSMO–PB

    Plasmid
    #169684
    Purpose
    COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104W
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169684-plasmid-map-sequence-id-338138

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  35. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54321-plasmid-map-sequence-id-84455

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  38. p240 pPr-CREM/Ins

    Plasmid
    #8405
    Purpose
    Androgen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.
    Depositor
    Jeffrey Green
    Article
    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56
    Insert
    probasin driven CREM with insulators
    Use
    Cre/Lox
    Expression
    Mammalian
    Mutation
    modified Cre
    Promoter
    Probasin promoter
    Available Since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 4921 - 4960 of 7945 results