We narrowed to 13,608 results for: crispr cas9

1. PX458-3xHA-SpCas9-HF1

   Plasmid

   #201952

   Purpose

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-sgR26-eCas9-T2A-BlastR

   Plasmid

   #172493

   Purpose

   For CRISPR-assisted HDR, Rosa26 locus

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-dCas9(sa)-VP64-AAV

   Plasmid

   #213035

   Purpose

   dCas9-VP64 expressed under control of TRE

   Depositor

   Nadav Ahituv

   Article

   Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

   Insert

   dCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and NLS-VP64

   Promoter

   CMV

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMMP-MCS-IRES-eGFP-P2A-iCas9

   Plasmid

   #178533

   Purpose

   For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

   Depositor

   Ryoji Yao

   Article

   Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

   Insert

   iCaspase9 (CASP9 Human)
   

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

   Plasmid

   #89793

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Q147L, CAA-->CTA

   Promoter

   hUbC

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RHO-IntC-dCas9C-VPR-polyA

   Plasmid

   #166692

   Purpose

   Express C-terminal part of split dCas9-VPR

   Depositor

   Stylianos Michalakis

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Insert

   IntC-dCas9C-VPR
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   short human rhodopsin promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

   Plasmid

   #112139

   Purpose

   Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

   Inserts

   sgRNA scaffold
   Human-codon-optimized NmeCas9
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U1a and U6

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

   Plasmid

   #133794

   Purpose

   U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   NmeCas9 and Nme2Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Amrani et al. Genome Biol…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid

    #92102

    Purpose

    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

    Plasmid

    #69219

    Purpose

    Expresses wild type SpCas9 fused to Zif268 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    Zif268 (Egr1 Mouse)
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

    Plasmid

    #60815

    Purpose

    dCas9 with single cysteine residue (M1C) for site-specific labelling

    Depositor

    Jennifer Doudna

    Article

    O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

    Insert

    dCas9 M1C D10A C80S H840A C574S
    

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    M1C D10A C80S H840A C574S

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

    Plasmid

    #228363

    Purpose

    mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

    Plasmid

    #171101

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Olig2 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

    Plasmid

    #171102

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Otx2 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

    Plasmid

    #101207

    Purpose

    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A and Q695A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid

    #101214

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C and K855A

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Hs.cargo(Cas9)

    Plasmid

    #174864

    Purpose

    Expresses human Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by HsPeg10 UTRs
    

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cp PS Intein dCas9-VPR

    Plasmid

    #242031

    Purpose

    Blue-light-controlled release of dCas9-VPR from the cytoplasm to the nucleus.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Cp PS Intein dCas9-VPR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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