-
Plasmid#127159PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotypeDepositorInsertIghg2c (Ighg2c Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP GluR2(R)
Plasmid#24001DepositorInsertGluR2 (Gria2 Rat)
UseTagsGluR1 signal peptide (aa1-22) and SEP (supereclip…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_MOKV_G
Plasmid#207294PurposeMammalian expression of Mokola virus G proteinDepositorInsertMOKV_G (G Mokola virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-BICRAL-FLAG-pgk-puro
Plasmid#107732PurposeTet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORFDepositorInsertBICRAL (BICRAL Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-TET
Plasmid#20909PurposepiggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)DepositorInsertGateway Destination Vector
UsepiggybacTagsIRES-bGeoExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTKLP-tetA
Plasmid#71325PurposetetA landing pad template plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry-IRES-Cre (AAV8)
Viral Prep#55632-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
iCAP-ROOT
Plasmid#192262PurposeAAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsidDepositorInsertSynthetic construct for ROOT VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse SLC KO Library
Pooled Library#141436PurposeExpresses gRNAs for knockout of the mouse Solute Carrier familyDepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYCN_P2A_Hygro_Barcode
Plasmid#120463PurposeBarcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYCN (MYCN Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCMV-flag-Caspase11
Plasmid#21145DepositorInsertCaspase11 (Casp4 Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8r
Plasmid#208318PurposeOverxpression MBP-TadA8rDepositorInsertTadA8r
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKRED
Plasmid#41062DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti_MCP-LSD1_Hygro
Plasmid#138457Purposelenti vector encoding the MS2-LSD1 with a 2A Hygro resistance markerDepositorInsertLSD1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1
Plasmid#32444PurposeMammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
UseTagsExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSCV IRES Luciferase
Plasmid#18760DepositorTypeEmpty backboneUseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA-flag-hKDM4A-polyA
Plasmid#101051Purposein vitro transcription (T7 promoter)DepositorInsertKDM4A (KDM4A Human)
UseTagsFlagExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-YU2gp160
Plasmid#100927PurposeMammalian expression plasmid for Env from the YU-2 HIV-1 isolateDepositorInsertHIV-1 (YU2) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
B-GRAM-W
Plasmid#211707PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cellsDepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,5)P2 Probe
Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-Pericam
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralTagsExpressionMammalianMutationPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKPI-MtLAP1
Plasmid#193521PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertsMtLAP1
DsRed
UseTagsExpressionPlantMutationPromoterATUBI10 and Medtr8g059790(KPI)Available sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRCherry2.1
Plasmid#108099PurposeLentiviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-m
Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorInsertABE8.20-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMD2.G-H8A/K47Q
Plasmid#207322PurposeVSV-G mutant with abolished receptor binding, but maintained fusogenic capacityDepositorInsertVSV-G (VSIVgp4 Vesicular Stomatitis Virus)
UseTagsExpressionMammalianMutationH8A/K47QPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV2)
Viral Prep#107738-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-p62
Plasmid#199780PurposeUsed for the expression of mCherry labelled p62 (SMC Internal No. 391).DepositorInsertp62 (NUP62 Human)
UseTags6x His, TEV, mCherryExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLEX_313 SLC31A1
Plasmid#184493PurposeLentivirus expression for SLC31A1 geneDepositorInsertSLC31A1 (SLC31A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Slow-FT
Plasmid#31915DepositorInsertSlow-FT
UseTagsExpressionMammalianMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoterPTight Tet-responsive PminCMVdAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry
Plasmid#221216Purposecontrol plasmid-part B of SPARK-OFFDepositorInsertNLS-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFR-mApple
Plasmid#203773PurposeNumber and brightness analysis of EGFR oligomerizationDepositorInsertEGFR (EGFR Human)
UseTagsmAppleExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.MaCPNS2
Plasmid#185137Purposenon-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS2 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-YE1-BE4max
Plasmid#198549PurposeExpresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG1.1
Plasmid#173685PurposeThe expression vector for genes of interest under CAG promoter.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xAP-1_RE::Renilla
Plasmid#178317PurposeAP-1 Renilla luciferase reporterDepositorInsertAP-1 Renilla Reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only