164,060 results

1. TFORF0055

   Plasmid

   #143201

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF678 (ZNF678 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0196

   Plasmid

   #143204

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF844 (ZNF844 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF2333

   Plasmid

   #142035

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF491 (ZNF491 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF2614

   Plasmid

   #142339

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF275 (ZNF275 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3542

   Plasmid

   #145018

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF720 (ZNF720 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF3153

   Plasmid

   #144629

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZFP2 (ZFP2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-Flpo

   Plasmid

   #174378

   Purpose

   also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Has Service

   AAV Retrograde

   Insert

   FLPo recombinase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMaCTag-P20

   Plasmid

   #120031

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   10xSuntag
   

   Use

   Pcr template

   Tags

   10xSuntag

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRlsinPGK_H2BGFP_WPRE

   Plasmid

   #91788

   Purpose

   Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.

   Depositor

   Beverly Torok-Storb

   Insert

   Human histone H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Jin Wang, Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. phCMV-coGALVenv-C4070A

    Plasmid

    #172217

    Purpose

    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

    Depositor

    Boris Fehse

    Article

    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

    Insert

    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Carries C-terminus of amphotropic MLV strain 4070…

    Promoter

    CMV immediate early promoter

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKIIa-mCherry (AAV1)

    Viral Prep

    #114469-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    mCherry

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

15. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIN4-EF2-ABCG2-IRES-Neo

    Plasmid

    #25983

    Depositor

    Ren-he Xu

    Article

    Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

    Insert

    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
    

    Use

    Lentiviral

    Tags

    EF1-apha promoter and IRES-Neo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. AnkyrinB-2XHA

    Plasmid

    #31057

    Depositor

    Vann Bennett

    Article

    Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

    Insert

    Ankyrin B (ANK2 Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. mNeonGreen-APEX3

    Plasmid

    #202590

    Purpose

    Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

    Depositor

    Reuben Harris

    Article

    Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

    Insert

    mNeonGreen-APEX3-V5 (APX1 )
    

    Use

    Retroviral

    Tags

    V5 epitope and mNeonGreen

    Expression

    Mammalian

    Mutation

    APEX2 L242A

    Promoter

    MESV LTR

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDONR221_SLC3A2

    Plasmid

    #132213

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC3A2 (SLC3A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-G-Creon shRNA[Control]

    Plasmid

    #181824

    Purpose

    This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. bact2-mKate2-P2A-TurboID-dVHH[GFP]

    Plasmid

    #163857

    Purpose

    Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID-VHH[EGFP]
    

    Use

    Zebrafish expression

    Tags

    mKate2-P2A-TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmcherry α-catenin WT

    Plasmid

    #178646

    Purpose

    express wild-type α-catenin

    Depositor

    Daniel Conway

    Article

    Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

    Insert

    α-catenin (CTNNA1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p1321 HPV-16 E6/E7

    Plasmid

    #8641

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E6/E7 (E7, E6 Human papillomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLenti6.3-sRGN3.1-BlastR

    Plasmid

    #220964

    Purpose

    Expresses human codon-optimized sRGN3.1

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    sRGN3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRIS-PITChv2-BSD-dTAG (BRD4)

    Plasmid

    #91792

    Purpose

    PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    BSD-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promoterless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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