We narrowed to 43,146 results for: cha

1. pGEX-6P-1-Cys-HA-Ubiquitin

   Plasmid

   #185356

   Purpose

   In vitro ubiquitination assays

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   Ubiquitin
   

   Use

   Tags

   GST, HA(x2)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LT2_32_PyruEth_X2site

   Plasmid

   #194428

   Purpose

   Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   PyruEth
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LT1_75_Donor_Tag_RPL13A-mTurq2

   Plasmid

   #194415

   Purpose

   Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   mTurquoise2 CDS
   

   Use

   Synthetic Biology; Used as donor dna in genome in…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pnYC-NpM-SpDcp2opt_1-242-E147Q_AD

   Plasmid

   #148546

   Purpose

   Bacterial Expression of SpDcp2opt_1-242-E147Q

   Depositor

   Elisa Izaurralde

   Article

   Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

   Insert

   SpDcp2opt_1-242-E147Q (dcp2 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFP4D42

   Plasmid

   #185840

   Purpose

   Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pILGFP10CF71

   Plasmid

   #185860

   Purpose

   Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pILGFP10CF72 (B9)

   Plasmid

   #185858

   Purpose

   Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pILGFP10CF72

   Plasmid

   #185857

   Purpose

   Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILGFP10E1B

   Plasmid

   #185856

   Purpose

   Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP10E1A

    Plasmid

    #185855

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pILGFP3A2 (1)

    Plasmid

    #185851

    Purpose

    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KBB441

    Plasmid

    #185131

    Purpose

    Swi6 full length with L->A mutated in nuclear localization signal

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    SWI6 deletion of amino acids 450-458

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB9341 (pgRNA_IX-1_NatMX)

    Plasmid

    #161593

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB9343 (pgRNA_XV-1_NatMX)

    Plasmid

    #161595

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KBB345

    Plasmid

    #185078

    Purpose

    rfa3D46 truncation fused to GFP

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA3
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Rfa3 truncation aa 1-46

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KBB419

    Plasmid

    #185089

    Purpose

    Swi6L-GFP expressing N-term 273 amino acids of Swi6

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 truncation at aa 273

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KBB354

    Plasmid

    #185082

    Purpose

    Swi6L-GFP expressin N-term 273 amino acids of Swi6

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 truncation aa 1-273

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KBB416

    Plasmid

    #185087

    Purpose

    Swi6L-GFP with nuclear export signal mutated to alanines

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 nuclear export signal L to A mutations

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KBB349

    Plasmid

    #185080

    Purpose

    rfa2D248 truncation fused to GFP

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA2
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Rfa2 truncation aa 1-247

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KBB353

    Plasmid

    #185081

    Purpose

    Swi6M-GFP expressing N-term 181 amino acids of Swi6

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 truncation aa 1-181

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. KBB484

    Plasmid

    #185132

    Purpose

    SWI6-GFP full length under control of GAL1 promotion

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KBB486

    Plasmid

    #185133

    Purpose

    Full length CRM1 fused to binding domain of two-hybrid plasmid

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    CRM1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KBB491

    Plasmid

    #185134

    Purpose

    Full length SWI6 fused to activation domain of two-hybrid plasmid

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. L1_UBE2:eG-Lt-Ck4

    Plasmid

    #136130

    Purpose

    L1 in position 4, mark cell outline with eGFP

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:eGFP-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PROM_MpNOP1

    Plasmid

    #136117

    Purpose

    PROM for cloning with 5UTR_MpNOP1

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_MpNOP1 Mapoly0008s0036
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. 3TERM_MpNOP1

    Plasmid

    #136119

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_MpNOP1 Mapoly0008s0036
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. L1_NOP1:mS-N7-Ck2

    Plasmid

    #136126

    Purpose

    L1 in position 2, example of weak promoter

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpNOP1:mScarletI-N7_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. L1_UBE2:mT-N7-Ck2

    Plasmid

    #136127

    Purpose

    L1 in position 2, control of expression

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mTurquoise2-N7_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. L1_UBE2:mT-N7-Ck3

    Plasmid

    #136128

    Purpose

    L1 in position 3, control of expression

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mTurquoise2-N7_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. L1_UBE2:eG-Lt-Ck3

    Plasmid

    #136129

    Purpose

    L1 in position 3, mark cell outline with eGFP

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:eGFP-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 5UTR_MpNOP1

    Plasmid

    #136103

    Purpose

    5UTR for cloning with PROM_MpNOP1

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    5UTR_MpNOP1 Mapoly0008s0036
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. CDS12_TP-MpSIG2

    Plasmid

    #136093

    Purpose

    Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    TP-MpSIG2, Mapoly0214s0004
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pCS2+-wbp2nlY282F

    Plasmid

    #172468

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY282F
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Tyrosine at aa282 to Phenylalanine

    Promoter

    SP6

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Ecm2 1-197

    Plasmid

    #169924

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-197
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Sc NybbR eRF1 (G180A)

    Plasmid

    #175415

    Purpose

    Encodes His-MBP-TEV-ybbR-G180A eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Mutation

    G180A

    Promoter

    T7

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2+-wbp2nlF127P

    Plasmid

    #174507

    Purpose

    expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlF127P
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Phenylalanine at aa127 to Proline

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCS2+-wbp2nlT45A

    Plasmid

    #172464

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlT45A
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Threonine at aa45 to Alanine

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2+-wbp2nlY55F

    Plasmid

    #172466

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY55F
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Tyrosine at aa55 to Phenylalanine

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Mdn1-FL

    Plasmid

    #171602

    Purpose

    Expresses full length S. pombe Mdn1 in insect cells.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    Mdn1
    

    Use

    Tags

    His6 tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pNTI763 p(Z)-mCherry + UBC9 sgRNA

    Plasmid

    #164918

    Purpose

    CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    P(Z)-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P(Z), synthetic promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge