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We narrowed to 45,354 results for: cha

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Showing: 5041 - 5080 of 45354 results

  7. p414-TEF1p-Cas9-CYC1t

    Plasmid
    #43802
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 promoter
    Available Since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43802-plasmid-map-sequence-id-333570

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  14. pMP11

    Plasmid
    #215559
    Purpose
    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin
    Depositor
    Brian Pfleger
    Article
    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10
    Inserts
    Cas9
    gRNA targeting pBR322 origin of replication
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23105 and tet
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    215559-plasmid-map-sequence-id-434132

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  17. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Expression
    Mammalian
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  18. p415-GalL-Cas9-CYC1t

    Plasmid
    #43804
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GalL
    Available Since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43804-plasmid-map-sequence-id-59154

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  19. ZJOM31

    Plasmid
    #133645
    Purpose
    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-TGL3-GFP (TGL3 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133645-plasmid-map-sequence-id-265890

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  22. R57C10-Flp2

    Plasmid
    #63172
    Purpose
    R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion
    Depositor
    Gerald Rubin
    Article
    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p
    Insert
    yeast wild type containing a Glycine at amino acid 5 of the protein sequence
    Expression
    Insect
    Available Since
    July 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pUltra-5HTP

    Plasmid
    #178042
    Purpose
    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    Expression
    Bacterial
    Mutation
    T107C, P254T, C255A
    Available Since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178042-plasmid-map-sequence-id-352270

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  25. pJP_Osc05

    Plasmid
    #229040
    Purpose
    Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.
    Depositor
    Yolanda Schaerli
    Article
    Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038
    Insert
    pBAD promoter
    Expression
    Bacterial
    Available Since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229040-plasmid-map-sequence-id-458369

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  33. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Article
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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  39. pFUGW-hGtACR2-EYFP

    Plasmid
    #67877
    Purpose
    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
    Depositor
    John Spudich
    Article
    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.
    Insert
    hGtACR2
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67877-plasmid-map-sequence-id-199004

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Showing: 5041 - 5080 of 45354 results