We narrowed to 44,527 results for: REP

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pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)

Tags

His6

Expression

Bacterial

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)

Plasmid

#145839

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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MSP2135

Plasmid

#72248

Purpose

Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, and D1135E

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-tdMCP-YFP

Plasmid

#99151

Purpose

Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

Dimeric MS2-coat binding protein

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDiGc

Plasmid

#59322

Purpose

Inducible expression of dsRed and constitutive expression of GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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Green Glifon50

Plasmid

#126206

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon50

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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hSyn1-sypHy-RGECO

Plasmid

#84078

Purpose

Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

Depositor

Juan Burrone

Article

Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

Insert

sypHy-RGECO

Expression

Mammalian

Promoter

hSyn1

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pEYFP-Mitotrap

Plasmid

#46942

Purpose

Expresses Mitotrap in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Mitotrap (MTOR Synthetic, Budding Yeast, Aequorea victoria, Human)

Tags

This is a fusion between a yeast mitochondrial ta…

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-wtSrc

Plasmid

#140294

Purpose

Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Tags

2X HA

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKIIa iChloC 2A tDimer

Plasmid

#66709

Purpose

Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

CaMKIIa

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-GPI

Plasmid

#65294

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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hGFAP-fLuc

Plasmid

#40589

Depositor

Albee Messing

Article

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

hGFAP promoter (GFAP Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Contains human GFAP promoter fragment (-2163 to +…

Promoter

GFAP promoter

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCDHblast MCSNard OST-LMNAd50

Plasmid

#22662

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

LaminAd50 (=Progerin)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pAd 2B Ngn3-I-nGFP

Plasmid

#19410

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

neurogenin 3 (Neurog3 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CFP

Plasmid

#14867

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

CFP

Tags

Myr/Palm

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pSFV-Helper1

Plasmid

#92073

Purpose

This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.

Use

Helper plasmid, for run-off transcription and rna…

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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MSP680

Plasmid

#65772

Purpose

Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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